摘要
目的系统调查关中平原蜱虫的种类及其地理分布特征,明确该地区蜱虫携带立克次体及嗜吞噬细胞无形体(AP)的流行特征及其遗传多样性。方法于2023年5月至2024年7月期间采集蜱虫样本,并提取DNA。采用巢式PCR法对立克次体和AP进行筛查,进一步进行系统进化分析。结果共计捕获10130只蜱虫,选择219只蜱虫进行病原携带检测。蜱种鉴定确认所检测的蜱虫为长角血蜱。病原体检测结果显示,立克次体和AP的携带率分别为8.68%和35.62%。系统进化分析表明,关中平原存在多种不同类型的立克次体和AP。其中一株立克次体的序列与已知参考序列均未形成聚类,提示该株可能属于一种新的变异株。结论关中平原地区的立克次体和AP携带率较高,且这两种病原体均可分为多种不同的进化支。该发现揭示了其致病潜力上可能的多样性,为进一步研究不同进化支的致病机制提供依据。
Objective To investigate the species and geographical distribution characteristics of ticks in the Guanzhong Plain,and to determine the endemic characteristics and genetic diversity of ticks carrying Rickettsia and Anaplasma phagocytophilum(AP).Methods Tick samples were collected from May 2023 to July 2024 and DNA was extracted.Nested PCR was used to screen for Rickettsia and AP for further phylogenetic analysis.Results A total of 10130 ticks were captured,and 219 ticks were selected for pathogen-carrying testing.Tick species identification confirmed that the detected ticks were Haemaphysalis longicornis.Pathogen detection results showed that the prevalence of Rickettsia and AP was 8.68%and 35.62%,respectively.Phylogenetic analysis showed that there were numerous variants of Rickettsia and AP in the Guanzhong Plain.The sequence of one Rickettsia strain did not cluster with the known reference sequence,suggesting that the strain may belong to a new variant strain.Conclusion The Guanzhong Plain has a high carrying rate of Rickettsia and AP,and these two pathogens can be divided into several different clades.This discovery reveals the potential diversity of their pathogenicity and provides a basis for further investigation of the pathogenicity of different clades.
作者
王玉华
刘嘉成
徐琳莉
贺真
陆振华
邵中军
刘昆
龙泳
WANG Yuhua;LIU Jiacheng;XU Linli;HE Zhen;LU Zhenhua;SHAO Zhongjun;LIU Kun;LONG Yong(Department of Epidemiology,Ministry of Education Key Laboratory of Hazard Assessment and Control in Special Operational Environment,School of Military Preventive Medicine,Air Force Medical University,Xi'an 710032,China)
出处
《空军军医大学学报》
2025年第5期591-597,604,共8页
Journal of Air Force Medical University
基金
国家自然科学基金(82273689)。
关键词
长角血蜱
一代测序
立克次体
嗜吞噬细胞无形体
关中平原
分子流行病学
Haemaphysalis longicornis
first-generation sequencing
Rickettsia
Anaplasma phagocytophilum
Guanzhong Plain
molecular epidemiology
作者简介
王玉华,硕士研究生,从事虫媒传染病研究,Tel:15129083221,E-mail:yhwang18@163.com;通信作者:龙泳,Tel:13572207057,E-mail:longyong@fmmu.edu.cn。
