摘要
为了筛选高效降解酱香型白酒窖底水红曲霉菌株,该研究采用传统培养分离法从酱香型白酒大曲中筛选红曲霉菌株,通过形态学观察及分子生物学技术对筛选菌株进行鉴定,利用生物量评估红曲霉的生长能力,考察筛选菌株发酵窖底水对有机污染物的降解效果及对产洛伐他汀和γ-氨基丁酸的影响并解析高附加值代谢产物合成机制。结果表明,分离筛选获得4株菌HSHM、AKHM、ZSHM和CMHM,分别被鉴定为红色红曲霉(Monascus ruber)、安卡红曲霉(Monascus anka)、紫色红曲霉(Monascus purpureus)和从毛红曲霉(Monascus pilosus)。菌株ZSHM生物量达(0.098±0.016) g/L,其在马铃薯葡萄糖肉汤培养基添加10%(V/V)窖底水中对生化需氧量(BOD5)和化学需氧量(CODcr)的去除率分别为56.76%、67.63%。菌株ZSHM在大米与高粱基质中发酵时,洛伐他汀、γ-氨基丁酸产量分别为(23.26±2.08) mg/100 g、(34.27±0.29) mg/100 g;(53.67±0.74) mg/100 g、(43.66±0.75) mg/100 g。在大米与高粱基质中添加10%(V/V)窖底水后,洛伐他汀产量显著降低至(16.56±0.71) mg/100 g、(35.16±2.08) mg/100 g(P<0.05),而γ-氨基丁酸产量则显著提升至(42.58±1.68) mg/100 g、(57.12±1.31) mg/100 g(P<0.05)。转录组分析表明,窖底水通过显著上调GAD、GDH2、ALDH及aofH基因表达,富集γ-氨基丁酸。
To obtain Monascus spp.with high efficiency in degrading pit bottom wastewater from sauce-flavor(Jiangxiangxing)Baijiu production,Monascus strains were screened from sauce-flavor Baijiu Daqu using traditional cultivation methods and identified through morphological observation and molecular biology techniques.The growth capacity of Monascus was evaluated with biomass.The degradation of organic pollutants and the effects of fermentation pit bottom wastewater on lovastatin andγ-aminobutyric acid production of screened strains were investigated,and the synthesis mechanism of high value-added metabolites was analyzed.The results showed that the isolated strains HSHM,AKHM,ZSHM and CMHM were identified as Monascus ruber,Monascus anka,Monascus purpureus,and Monascus pilosus,respectively.Strain ZSHM exhibited the highest biomass(0.098±0.016 g/L)and achieved removal rates of 56.76%biochemical oxygen demand(BOD5)and 67.63%chemical oxygen demand(CODcr)indextrose broth medium supplemented with 10%(V/V)pit bottom wastewater.When strain ZSHM was fermented in rice and sorghum substrates,the yields of lovastatin andγ-aminobutyric acid were(23.26±2.08)mg/100 g,(34.27±0.29)mg/100 g and(53.67±0.74)mg/100 g,and(43.66±0.75)mg/100 g,respectively.After adding pit bottom wastewater 10%(V/V)to rice and sorghum substrate,lovastatin yield were significantly reduced to(16.56±0.71)mg/100 g and(35.16±2.08)mg/100 g(P<0.05),andγ-aminobutyric acid yield were significantly increased to(42.58±1.68)mg/100 g and(57.12±1.31)mg/100 g(P<0.05).Transcriptomic analysis revealed that pit bottom wastewater enrichedγ-aminobutyric acid by significantly up-regulating genes expression of GAD,GDH2,ALDH,and aofH.
作者
李炫辰
杨欣
程迈
罗玥娜
周剑丽
邱树毅
LI Xuanchen;YANG Xin;CHENG Mai;LUO Yuena;ZHOU Jianli;QIU Shuyi(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Province Key Laboratory of Fermentation Engineering and Biopharmacy,Guizhou University,Guiyang 550025,China;Guizhou Longqianwei Liquor Co.,Ltd.,Anshun 561100,China)
出处
《中国酿造》
北大核心
2025年第5期104-112,共9页
China Brewing
基金
国家自然科学基金地区基金项目(32360228)。
关键词
窖底水
红曲霉
分离鉴定
降解处理
洛伐他汀
Γ-氨基丁酸
pit bottom wastewater
Monascus
isolation and identification
degradation treatment
lovastatin
γ-aminobutyric acid
作者简介
李炫辰(1999-),男,硕士研究生,研究方向为食品微生物和发酵工程;通讯作者:周剑丽(1982-),女,副教授,博士,研究方向为食品微生物和发酵工程。
