摘要
通过对螺旋藻进行不同的光照培养处理,获得了微观形态和光合色素含量不同的微藻,分析其光谱特性,探究能否提高生物量光密度检测方法的准确性。结果表明,一定范围内,藻液光密度与藻丝体长度呈负相关,与光合色素含量呈正相关。通过对藻丝体进行超声均一化处理(400 W间歇超声处理1 min)、筛选光密度检测波长(520 nm),可以减少因微藻形态和光合色素含量造成的吸收光谱差异,提高生物量检测的准确性。
Through different light culture treatment of Arthrospira platensis,microalgae with different microscopic morphology and photosynthetic pigment content were obtained,and their spectral characteristics were analyzed to explore whether the accuracy of the biomass photodensity detection method could be improved.The results showed that the light density of algal liquid was negatively correlated with the length of algal filament and positively correlated with the content of photosynthetic pigment in a certain range.Through ultrasonic homogenization of algal filaments(400 W intermittent ultrasonic treatment for 1 min)and screening of optical density detection wavelength(520 nm),the differences in absorption spectra caused by microalgae morphology and photosynthetic pigment content could be reduced,and the accuracy of biomass detection couldbe improved.
作者
王晓旭
毛瑞鑫
吴浩
艾为党
刘力涛
魏学军
Wang Xiaoxu;Mao Ruixin;Wu Hao;Ai Weidang;Liu Litao;Wei Xuejun(School of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056038,Hebei,China;National Key Laboratory of Human Factors Engineering,Beijing 100094,China;China Astronaut Research and Training Center,Beijing 100094,China)
出处
《农业技术与装备》
2025年第4期3-7,共5页
Agricultural Technology & Equipment
基金
人因工程全国重点试验室基金资助(GJSD22002)。
关键词
光密度
吸收光谱
生物量
藻丝体形态
光合色素
optical density
absorption spectrum
biomass
algal filament morphology
photosynthetic pigments
作者简介
王晓旭(1999-),男,蒙古族,河北隆化人,硕士研究生在读,研究方向为藻类培养技术。
