摘要
目的:研究PD-L2基因敲除对C57BL/6小鼠脾脏免疫表型的影响。方法:连续称量雌性和雄性野生型(WT)和PD-L2基因敲除(KO)小鼠3~12周的体质量。HE染色观察4组小鼠心、肝、脾、肺、肾、胸腺、卵巢、子宫和睾丸组织结构差异;脲酶紫外速率法测量小鼠血清中尿素水平;肌氨酸氧化酶法检测小鼠血清肌酐浓度;流式细胞术检测小鼠脾脏各免疫细胞比例及细胞因子差异。结果:PD-L2基因敲除对小鼠体质量及各器官结构无明显影响。雌性KO小鼠血清尿素和肌酐浓度均高于WT小鼠,但雄性之间差异无统计学意义。雌性KO小鼠脾脏CD4^(+)T细胞、CD8^(+)T细胞、Treg、NK细胞和树突状细胞(DC)较WT小鼠比例降低,而Tfh、B细胞、浆细胞、na?ve B细胞和B2细胞比例升高,IL-17、IL-6和IL-22等促炎细胞因子增加,而IL-10、IL-4等抑炎细胞因子减少,雄性小鼠之间差异不明显或无差异。结论:PD-L2基因敲除主要影响雌性小鼠外周免疫表型,敲除后小鼠炎症反应激活,免疫抑制减弱。
Objective:To investigate impact of PD-L2 gene knockout on splenic immune phenotype of C57BL/6 mice.Methods:Body weight of female and male wild-type(WT)and PD-L2 gene knockout(KO)mice aged 3~12 weeks were continuously measured.Tissue structure differences in heart,liver,spleen,lung,kidney,thymus,ovary,uterus and testis of four groups of mice were observed by HE staining.Urease UV rate method was employed to measure urea level in mouse serum,and creatinine concentration in serum was determined by creatine oxidase method.Flow cytometry was used to assess proportions of immune cells and cytokine differences in spleens of mice.Results:PD-L2 gene knockout had no apparent effect on body weight or organ structure of mice.Serum urea and creatinine levels were higher in female KO mice compared to WT mice,but no significant differences were observed among males.Female KO mice exhibited reduced proportions of CD4^(+)T cells,CD8^(+)T cells,Treg,NK cells and dendritic cells(DC)in spleen compared to WT mice.Conversely,proportions of Tfh,B cells,plasma cells,naïve B cells and B2 cells were elevated,proinflammatory cytokines such as IL-17,IL-6 and IL-22 were increased,while anti-inflammatory cytokines like IL-10 and IL-4 were decreased.Differences among male mice were either not significant or absent.Conclusion:PD-L2 gene knockout primarily affects splenic immune phenotype of female mice,leading to increased inflammatory responses and weakened immune suppression after knockout.
作者
杨艳红
宋赛哲
刘翠平
YANG Yanhong;SONG Saizhe;LIU Cuiping(Jiangsu Institute of Clinical Immunology,the First Affiliated Hospital of Soochow University,Suzhou 215021,China;Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Soochow University,Suzhou 215004,China)
出处
《中国免疫学杂志》
北大核心
2025年第5期1048-1054,共7页
Chinese Journal of Immunology
基金
苏州市姑苏卫生人才计划培养项目(GSWS2020011)
苏州市科技计划项目(SKY2023138)。
关键词
PD-L2
基因敲除
组织结构
免疫表型
炎症反应
PD-L2
Gene knockout
Tissue structure
Immune phenotype
Inflammatory response
作者简介
杨艳红,女,在读硕士,主要从事免疫与生殖方面的研究,E-mail:yang18806298619@126.com;通信作者:刘翠平,女,博士,教授,主要从事自身免疫疾病方面的研究,E-mail:liucuiping1980@126.com。
