摘要
探究纯菌混合发酵康普茶代谢产物的组成,并预测其调节代谢综合征的潜在功效,为功能性康普茶饮品的开发利用提供理论依据。通过超高效液相色谱-飞行时间质谱技术对新型康普茶发酵产物进行非靶向代谢组学研究,全面鉴定代谢产物组分,利用网络药理学、分子对接技术与体外活性测定解析与验证关键化合物调节代谢综合征的作用机制。从新型康普茶发酵产物中共鉴定到1148种产物,基于主成分分析和偏最小二乘判别分析结果共筛选到68种显著上调代谢产物。网络药理学分析共筛选出10种关键功效成分、382个化合物靶点以及155个疾病交集靶点。基因本体功能和京都基因和基因组百科全书通路富集分析分别得到生物过程1490条、细胞组分96条、分子功能145条和通路172条。网络拓扑学分析共得到INS、IL6、LEP、TNF、ALB、STAT3、IL1B、TP538个核心靶点,分子对接实验结果表明山柰酚-7-新橘皮糖苷、棉籽糖、西蒙得木素、花旗松素与各受体蛋白结合效果最好。体外活性测定结果表明新型康普茶具有较好的体外1,1-二苯基-2-三硝基苯肼自由基、2,2′-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基的清除活性以及α-淀粉酶、α-葡萄糖苷酶、胰脂肪酶的抑制活性。新型康普茶发酵产生多种活性代谢产物,对代谢综合征的调节作用机制具有多成分、多靶点和多通路的特点,或可成为调节代谢综合征的功能性饮品。
Objective:To explore the composition of metabolites in Kombucha fermented by a mixed culture of Acetobacter xylinus,Lactobacillus fermentum and Zygosaccharomyces bailii and to predict its potential active compounds with regulatory effects on metabolic syndrome so as to provide a theoretical basis for the development and utilization of functional Kombucha beverages.Methods:Non-targeted metabolomics based on ultra-high performance liquid chromatography-time-of-flight mass spectrometry(UPLC-TOF-MS)was employed to identify the metabolites in the Kombucha,and the mechanism by which the key compounds regulate metabolic syndrome was analyzed by molecular docking and network pharmacology and verified by in vitro activity assays.Results:A total of 1148 metabolites were identified from the novel Kombucha.The results of principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)indicated that 68 metabolites were significantly up-regulated.Network pharmacology analysis selected 10 key functional components,382 compound targets and 155 disease intersection targets.Gene Ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis identified 1490 biological processes,96 cellular components,145 molecular functions and 172 pathways.Network topological analysis identified eight core targets including INS,IL6,LEP,TNF,ALB,STAT3,IL1B,and TP53.The results of molecular docking showed that kaempferol-7-neohesperidin,raffinose,simmondsin,and taxifolin had the best binding effect to eight receptor proteins.The results of in vitro activity assays showed that the Kombucha had good scavenging activity against 1,1-diphenyl-2-picryl hydrazyl(DPPH)radical and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical,as well as inhibitory activity againstα-amylase,α-glucosidase and pancreatic lipase.Conclusion:The novel Kombucha contained a variety of active metabolites,which exertedvregulatory effects on metabolic syndrome through a multi-component,multi-target and multi-pathway mechanism.The Kombucha may be a functional beverage for regulating metabolic syndrome.
作者
陈家领
王菁
林思敏
钱雪芹
阮晖
CHEN Jialing;WANG Jing;LIN Simin;QIAN Xueqin;RUAN Hui(College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;Pelaya Cosmetics Co.Ltd.,Hangzhou 310000,China)
出处
《食品科学》
北大核心
2025年第8期247-258,共12页
Food Science
基金
浙江大学与珀莱雅化妆品股份有限公司合作项目(2023330004003652)。
关键词
康普茶
代谢综合征
代谢组学
网络药理学
分子对接
Kombucha
metabolic syndrome
metabolomics
network pharmacology
molecular docking
作者简介
第一作者:陈家领(1999-)(ORCID:0009-0003-8519-179X),女,硕士,研究方向为功能微生物发酵与功能性食品开发。E-mail:chenjialing1999@zju.edu.cn;通信作者:阮晖(1968-)(ORCID:0000-0002-1340-7489),男,副教授,博士,研究方向为功能微生物发酵与功效组学解析。E-mail:andrewhruan@zju.edu.cn。
