摘要
本研究旨在对比分析青藏高原寒区3种十字花科人工牧草康奈尔净碳水化合物-蛋白质体系(CNCPS)蛋白组分及体外发酵参数,为十字花科人工牧草的合理栽培及其饲用价值优化提供科学依据。选择青藏高原寒区常见的3种十字花科人工牧草(华油杂62油菜、饲油2号油菜和芸芥)为研究对象,测定3种牧草的常规营养成分,采用CNCPS对其蛋白组分进行分析,并结合体外瘤胃发酵技术综合评定3种牧草的饲用价值。结果表明,华油杂62油菜的粗蛋白(CP)含量显著高于饲油2号油菜和芸芥(P<0.05),华油杂62油菜和芸芥的中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)差异不显著(P>0.05),但均显著高于饲油2号油菜(P<0.05)。华油杂62油菜的中速降解蛋白(PB_2)显著高于饲油2号油菜和芸芥(P<0.05)。华油杂62油菜的真蛋白(TCP)和可利用真蛋白(ATCP)均最高。3种牧草可利用粗蛋白(uCP)含量差异均不显著(P>0.05),华油杂62油菜的pH和微生物蛋白(MCP)含量最高,均显著高于饲油2号油菜(P<0.05)。3种牧草间乙酸(AA)、丙酸(PA)、异丁酸(IBA)、丁酸(BA)、异戊酸(IVA)、戊酸(VA)和总挥发性脂肪酸(TVFA)含量差异均不显著(P>0.05)。综上所述,华油杂62油菜饲用价值较高,可推广为青藏高原寒区十字花科主要人工牧草。
The purpose of this experiment was to compare and analyze the protein components of artificially cultivated cruciferous herbage by Cor-nellnet carbohydrate and protein system(CNCPS),and in vitro fermentation parameters of these for-ages in the high-cold region of the Qinghai-Xizang Plateau,aiming to provide a scientific basis for the rationally using of cultivation Crucif-erous forage grass and preferably optimizing the value of feeding.Three kinds of Cruciferous herb-age(Brassica napus L.huayouza 62,Brassica napus L.siyou 2 and Eruca sativa Mill.)commonly were selected as the research objects,and determined the nutrient composition of the forage,and the protein components of the three forages were analyzed by Cornell-net carbohydrate and protein system(CNCPS),and the feed value of the three forages was comprehensively evaluated by rumen fermentation in vitro.The results showed that the Brassica napus L.huayouza 62 had the highest crude protein,which was significantly higher than that of Brassica napus L.siyou 2 and Eruca sativa Mill.(P<0.05),and there was no significant difference in NDF and ADF content between Brassica napus L.huayouza 62 and Eruca sativa Mill.(P>0.05),but both were significantly higher than those of Brassica napus L.siyou 2.Brassica napus L.huayouza 62 was significant higher than that of Brassica napus L.siyou 2 and Eruca sativa Mill.in PB2(P<0.05).The TCP and ATCP of Brassica napus L.huayouza 62 were the highest.There was no significant difference in uCP content among Brassica napus L.huayouza 62,Brassica napus L.siyou 2.and Eruca sativa Mill.(P>0.05),while the pH value and MCP content of Brassica napus L.huayouza 62 were the highest,which were significantly higher than those of Brassica napus L.siyou 2(P<0.05).There were no significant differences in the contents of acetic acid,propionic acid,isobutyric acid,butyric acid,isovaleric acid,valeric acid and total volatile fatty acids among three forages(P>0.05).In conclusion,Bras-sica napus L.huayouza 62 has a high feed value,which could be promoted as the main artificial cruciferous herbage of the cruciferous family in the high-cold region of the Qinghai-Xizang Plateau,and play a role in the development of local husbandry industry.
作者
刘忠起
靳倩伶
张群英
周学丽
钟荣珍
王雷
杜雪燕
杨英魁
拜彬强
郝力壮
LIU Zhongqi;JIN Qianling;ZHANG Qunying;ZHOU Xueli;ZHONG Rongzhen;WANG Lei;DU Xueyan;YANG Yingkui;BAI Binqiang;HAO Lizhuang(Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province,Qinghai University,Qinghai Xining 810016,China;Qinghai Grassland Station,Qinghai Xining 810006,China;Trans-Regional Cooperation Science and Technology Innovation Center for Feed Processing and Precision Feeding of Grass-Fed Livestock,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Jilin Changchun 130102,China;Qinghai Province Forage Technology Extension Station,Qinghai Xining 810000,China)
出处
《饲料工业》
北大核心
2025年第6期16-22,共7页
Feed Industry
基金
国家重点研发子课题[2022YFD1302103、2022YFD1602301]
国家自然科学基金地方联合基金项目[U20A2002]
青海省高原放牧家畜动物营养与饲料科学重点实验室[重点实验室评估奖励项目:1-8]。
作者简介
刘忠起,硕士,研究方向为动物营养与饲料科学;通信作者:拜彬强,博士,副教授,硕士生导师;通信作者:郝力壮,博士,研究员,博士生导师。
