摘要
为探究褪黑素处理对采后‘桃形李’果实品质及花色苷代谢的影响,以七成熟嵊州‘桃形李’为材料,将采后果实在蒸馏水(对照)和0.1 mmol·L^(-1)褪黑素溶液中浸泡1 h后取出晾干,于室温(25±1)℃和75%~80%相对湿度条件下保存18 d,研究褪黑素处理对果实色泽、硬度、腐烂率、呼吸速率、乙烯释放速率、可溶性糖含量、花色苷代谢的影响。结果表明,褪黑素处理抑制了果实软化与腐烂,延缓了果实转色,降低了呼吸速率和乙烯释放速率,并推迟了乙烯释放高峰,延缓了果实糖类物质的积累。褪黑素处理可通过抑制PsCHI基因表达以及肉桂酸羧化酶(C4H)、4–香豆酰CoA连接酶(4-CL)、查尔酮异构酶(CHI)、黄烷酮羟化酶(F3H)、二氢黄酮醇还原酶(DFR)和花青素合成酶(ANS)的活性,延缓了花色苷含量增加。在贮藏后期,褪黑素处理显著上调了果实结构基因Ps CHS、PsF3H、PsDFR、PsANS与PsUFGT(中后期)的表达量,提高了果实C4H、4-CL、F3H、DFR、ANS的酶活性,促进了花色苷的合成。因此,褪黑素处理不仅延缓了果实的成熟进程,而且提高了果实成熟后期花色苷的合成,进而有利于延长果实的货架期,并增加果实完熟后的花色苷积累量从而提高果品的营养价值。
This study aimed to explore the effects of exogenous melatonin treatment on the fruit quality and anthocyanin metabolism of‘Taoxingli’plum fruit during storage.The‘Taoxingli’plum fruit about 70%maturity were harvested,and were soaked in water(as the control)or 0.1 mmol·L^(-1)melatonin solution for 1h and then dried in air.The fruit were stored at room temperature(25±1)℃ and relative humidity of 75%–80% for 18 days,and the changes in color,firmness,decay rate,respiration rate,ethylene release,soluble solids content,and anthocyanin metabolism of the fruit were investigated during storage.The results showed that melatonin treatment inhibited the softening and decay of fruit,delayed in the color transformation,reduced respiration rate and ethylene release,postponed the ethylene release peak and the accumulation of carbohydrate in the fruit compared to the control.Melatonin treatment slowed down the anthocyanin accumulation in the fruit by inhibiting the expression of PsCHI gene and the activities of the enzymes involved in anthocyanin synthesis,including cinnamate carboxylase(C4H),4-coumaryl CoA ligase(4-CL),chalcone isomerase(CHI),flavanone hydroxylase(F3H),dihydroflavonol reductase(DFR)and anthocyanin synthetase(ANS)at the beginning time of storage,whereas at the later stage of storage,melatonin treatment significantly up-regulated the expressions of the structural genes including PsCHS,PsF3H,PsDFR,PsANS and PsUFGT related to synthesis of anthocyanins along with increases in the activities of C4H,4-CL,F3H,DFR and ANS,which could promote the synthesis of anthocyanins.Therefore,melatonin treatment not only delayed the process of fruit ripening,but also was of benefit to the accumulation of anthocyanins in the full-ripening fruit,which could be contributed to extending the shelf life and improving the nutritional value of fruit.
作者
许桐
王越
吴丽娜
张航
尹立来
徐柯宇
郑小林
XU Tong;WANG Yue;WU Lina;ZHANG Hang;YIN Lilai;XU Keyu;ZHENG Xiaolin(School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China;Hangzhou Lin'an District Center for Disease Control and Prevention(Hangzhou Lin’an District Health Supervision Institute),Hangzhou 311300,China)
出处
《园艺学报》
北大核心
2025年第2期395-405,共11页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(32072280)。
关键词
李
果实
褪黑素
花色苷代谢
品质
采后
plum
fruit
melatonin
anthocyanin metabolism
quality
postharvest
作者简介
通信作者:郑小林(E-mail:zheng9393@163.com)。
