摘要
目的对冬季采集的1040名健康献浆员血浆样本进行血浆常规检测、SARS-CoV-2核酸检测及呼吸道病原微生物核酸检测分析。方法收集2020年冬季云梦单采血浆站采集的1040名健康献浆员血浆样本。对原料血浆进行常规项目检测,按照对应检测试剂盒说明书要求对HBV/HCV/HIV、SARS-CoV-2及22种呼吸道病原微生物进行核酸检测并分析。结果1040份血浆样本中常规项目检测均合格,符合中国药典(2020版)要求。1040份血浆样本SARS-CoV-2核酸均为阴性。筛查出29份呼吸道病原微生物核酸阳性样本,阳性率2.79%(29/1040)。其中单纯病毒感染21例,包括冠状病毒亚型感染17例、副流感病毒2型3例、博卡病毒1例;病毒和细菌混合感染8例,其中4例为病毒感染合并百日咳鲍特菌。29份阳性样本分布在不同的年龄段,其中31~40岁2份(1.96%,2/102),41~50岁3份(1.59%,3/189),51~55岁5份(1.94%,5/257),56~60岁19份(4.59%,19/414)。56~60岁年龄段血浆样本占比最高,达39.81%(414/1040),且此年龄段感染比例也最高。结论秋冬季节采浆时,56~60岁年龄段献浆员应在中国药典(2020版)要求的病毒指标基础上增加呼吸道病原微生物核酸检测;建议增加合并血浆和血液制品成品呼吸道病原微生物核酸检测。
Objective To perform routine plasma test,SARS-CoV-2 nucleic acid test,and respiratory pathogenic microorganism nucleic acid test on plasma samples collected from 1040 healthy plasma donors in winter.MethodsPlasma samples were collected from 1040 healthy plasma donors at Yunmeng Plasma Collection Station in the winter of 2020.Routine plasma test,HBV/HCV/HIV nucleic acid test,SARS-CoV-2 nucleic acid test,and 22 respiratory pathogenic microorganism nucleic acid test were performed to analyze the quality of blood plasmas.ResultsAll plasma samples were qualified in the routine tests,meeting the requirements of the Chinese Pharmacopoeia,and tested negative for SARS-CoV-2 nucleic acid.Respiratory pathogenic microorganism nucleic acids were detected in 29 samples with a positive rate of 2.79%(29/1040).There were 21 cases of simple virus infections,including 17 cases of coronavirus subtype infection,three cases of parainfluenza virus type 2 infection,and one case of human bocavirus infection.Eight cases were mixed infections of viruses and bacteria,four of which were viral infection combined with Bordetella pertussis.The 29 positive samples were collected from people of different age groups,including two from 31-40 years old(1.96%,2/102),three from 41-50 years old(1.59%,3/189),five from 51-55 years old(1.94%,5/257),and 19 from 56-60 years old(4.59%,19/414).Samples from the people aged 56-60 years accounted for the most(39.81%,414/1040),as well as the infection rate in this age group.ConclusionsIn autumn and winter,respiratory pathogenic microorganism nucleic acid test should be performed when collecting plasma samples from donors aged 56-60 years in addition to meeting the requirements of the Chinese Pharmacopoeia.It is also suggested to conduct respiratory pathogenic microorganism nucleic acid test on pooled plasma and blood products.
作者
王月
成立
刘莹
龚钦
童剑啸
赵传波
郭佳茹
罗艳
张金
Wang Yue;Cheng Li;Liu Ying;Gong Qin;Tong Jianxiao;Zhao Chuanbo;Guo Jiaru;Luo Yan;Zhang Jin(Ouality Control Department,Sinopharm Wuhan Biopharmaceuticals Co.,Ltd.,Wuhan 430207,China;Inspection Center of Hubei Medical Products Adminstration(Hubei Center for Vaccine Inspection),Wuhan 430077,China)
出处
《中华微生物学和免疫学杂志》
北大核心
2025年第2期141-148,共8页
Chinese Journal of Microbiology and Immunology
关键词
病原微生物
病毒感染
细菌感染
献浆员
Pathogenic microorganism
Virus infection
Bacterial infection
Plasma donor
作者简介
通信作者:张金,Email:lzs_zhangjin@163.com;王月,同等贡献;成立,同等贡献。
