摘要
为探究高温胁迫下摄食高水平淀粉饲料对大口黑鲈(Micropterus salmoides)生长性能、抗氧化能力、免疫能力和糖代谢的影响,实验设置对照组(NC)、高温组(HT)和高温高淀粉组(HH),分别投喂10%、10%和15%淀粉含量的等氮等脂配合饲料,养殖温度为28、32和32℃,选取初始质量为(8.81±0.22) g大口黑鲈随机分为三组,每组设3个重复,每个重复为40尾,饲养周期为60 d。结果显示,HH组与HT组的大口黑鲈增重率、特定生长率和饲料系数差异不显著,但两组增重率、特定生长率均显著低于NC组,饵料系数均显著高于NC组,HH组肝体比和脏体比显著高于HT组,HT组肝体比和脏体比与NC组无显著差异。HH组肝糖原显著高于NC和HT组,血糖显著低于HT组,而与NC组无显著差异。HH组肝脏酸性磷酸酶(ACP)与HT组无显著差异,碱性磷酸酶(AKP)、溶菌酶(LZM)、过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物(GSH-PX)活性显著低于HT组,丙二醛(MDA)活性显著高于HT组。HT组肝脏ACP、AKP、LZM、CAT和GSH-PX活性显著低于NC组,MDA活性显著高于NC组,T-SOD活性与NC组无显著差异。与NC组相比,HT组肝脏丙酮酸激酶(Pk)基因表达量显著上升,己糖激酶(Hk)、磷酸烯醇式丙酮酸羧激酶(Pepck)和葡萄糖-6-磷酸酶(G6pase)基因表达量显著下降。HH组中的肝脏Pepck基因表达量显著高于HT组,G6pase、Pk和Hk基因表达量与HT组无显著差异。研究结果表明大口黑鲈在高温胁迫下摄食高水平淀粉饲料会降低其生长性能、抗氧化能力和非特异性免疫能力,提高饲料系数、肝体比、脏体比和肝糖原含量。
The control group(NC),high temperature group(HT)and high temperature and high starch group(HH)were fed with 10%,10%and 15%starch content iso-nitrogen and other lipids,respectively to investigate the effects of a high-level starch diet on the growth performance,antioxidant capacity,immune capacity,and glucose metabolism of Macrocephalus salmoides under high-temperature stress.The culture temperature was 28℃,32℃and 32℃.The initial mass of(8.81±0.22)g M.salmoides was randomly divided into three groups with three replicates in each group and each replicate containing 40 bass.The feeding cycle lasted for 60 days.The results showed that no significant differences in weight gain rate,specific growth rate,and feed coefficient between the HH group and HT group were detected.However,the weight gain rate and specific growth rate in the two groups were significantly lower than those in the NC group,and the feed coefficient was significantly higher than that in the NC group.The hepatosomatic index and visceral somatic index in the HH group were significantly higher than those in the HT group.Still,the hepatosomatic index and visceral somatic index in the HT group were not significantly different from those in the NC group.The liver glycogen of the HH group was significantly higher than that of the NC and HT groups,and the blood glucose of the HH group was significantly lower than that of the HT group.Still,there was no significant difference between HH group and NC group.There were no significant differences between liver acid phosphatase(ACP)and HT group.Still,the activities of alkaline phosphatase(AKP),lysozyme(LZM),catalase(CAT),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-PX)in HH group were significantly lower than those in HT group.The malondialdehyde(MDA)activity was significantly higher than that of the HT group.The liver ACP,AKP,LZM,CAT,and GSH-PX activities of HT group were significantly lower than those of NC group,MDA activity was significantly higher than that of NC group,and T-SOD activity had no significant difference between HT group and NC group.Compared with the NC group,liver pyruvate kinase(Pk)gene expression significantly increased in the HT group.The expression of hexokinase(Hk),phosphoenolpyruvate carboxykinase(Pepck)gene,and glucose-6-phosphatase(G6pase)were significantly decreased.The liver Pepck gene expression in the HH group was significantly higher than in the HT group,but the G6pase,Pk,and Hk gene expressions were not significantly different from those in the HT group.The results showed that the growth performance,antioxidant capacity,and immune capacity of the M.salmoides were decreased by feeding a high-level starch diet under high-temperature stress.In contrast,the feed coefficient,hepatosomatic index,visceral somatic index,and liver glycogen content were increased.
作者
阎瀚威
杜金星
宋红梅
李铭
朱涛
雷彩霞
王耀华
李胜杰
YAN Hanwei;DU Jinxing;SONG Hongmei;LI Ming;ZHU Tao;LEI Caixia;WANG Yaohua;LI Shengjie(College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation,Ministry of Agriculture and Rural Affairs,Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;Guangzhou Liankun Biotechnology Co.LTD,Guangzhou 510380,China)
出处
《淡水渔业》
北大核心
2025年第2期16-25,共10页
Freshwater Fisheries
基金
广州市科技计划项目(202201010211)
广东省乡村振兴战略专项(2022SPY00003)。
作者简介
第一作者:阎瀚威(1999-),男,硕士研究生,专业方向为水产动物遗传育种。E-mail:yanhanwei0128@163.com;通讯作者:李胜杰。E-mail:ssjjli@163.com。
