摘要
目的建立一种快速测定全血中总溴的电感耦合等离子体质谱方法,为职业人群溴接触监测提供技术支撑。方法于2023年9月,手肘部取0.25 ml全血样加0.25%四甲基氢氧化铵溶液定容至5.0 ml,超声均匀后,以铑溶液为在线内标溶液,采用电感耦合等离子体质谱法进行定量测定。结果全血中总溴在0~10.00 mg/L内线性关系良好,相关系数(r)>0.999。该方法检出限为0.07 mg/L,定量限为0.22 mg/L。全血中总溴回收率范围为95.5%~102.9%,相对标准偏差范围为3.1%~4.7%(n=7)。结论本次研究建立一种快速测定全血中总溴的电感耦合等离子体质谱法,方法准确、简单、快速、自动化程度高,可用于职业接触人群全血中总溴的测定。
Objective To establish an inductively coupled plasma mass spectrometry(ICP-MS)method for the rapid determination of total bromine in whole blood,and to provide technical support for the monitoring of bromine exposure in occupational populations.MethodsIn September 2023,0.25 ml of whole blood sample was added with 0.25%tetramethylammonium hydroxide solution to a volume of 5.0 ml,ultrasonicated and homogenized,and then quantified by ICP-MS with rhodium solution as the online internal standard solution.ResultsThe linear relationship of bromine in blood was good within the range of 0~10.00 mg/L,with a correlation coefficient(r)>0.999.The limit of detection of the method was 0.07 mg/L,and the quantification limit was 0.22 mg/L.The recoveries of the total bromine in whole blood were in the range of 95.5%-102.9%,and the relative standard deviations(RSDs)were in the range of 3.1%-4.7%(n=7).ConclusionAn ICP-MS method was established for the rapid determination of total bromine in whole blood,which is accurate,simple,rapid,highly automated,and can be used for the determination of total bromine in whole blood of occupationally exposed people.
作者
龚燕
吴宇伉
孟元华
陈晓峰
Gong Yan;Wu Yukang;Meng Yuanhua;Chen Xiaofeng(The Affiliated Wuxi Center for Disease Control and Prevention of Nanjing Medical University,Wuxi Center for Disease Control and Prevention,Physical and Chemical Testing Department,Wuxi 214023,China)
出处
《中华劳动卫生职业病杂志》
北大核心
2025年第1期68-71,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
武汉科技大学职业危害识别与控制湖北省重点实验室开放基金(OHIC2022Y03)
无锡市科协软课题(KX-23-C134)
江苏省职业健康科研项目(JSZJ20231102)
无锡市卫健委医学重点学科项目:无锡市公共卫生中心(LCZX2021006)
无锡市医学创新团队(CXTD2021004)。
关键词
串联质谱法
血清
溴
电感耦合等离子体质谱
超声辅助
Mass spectometry
Whole blood
Bromine
Inductively coupled plasma mass spectrometry
Ultrasonic-assisted
作者简介
通信作者:孟元华,E-mail:mengyuanhua1010@126.com。
