摘要
选择36只45d左右、健康、体质量(6.30±0.54)kg的广丰山羊羔羊,随机分为3组,每组12只,对照组饲喂基础日粮(CON),试验组在基础日粮中分别添加100 mg/kg(Q100)和200 mg/kg(Q200)的槲皮素,饲喂15 d,在60日龄断奶,断奶后再饲喂15 d,分别于断奶前15 d、断奶当日、断奶后15 d测定羔羊体尺指标,同时,测定断奶当日和断奶15 d羔羊血清生化、免疫和抗氧化指标。结果表明:1)基础日粮添加槲皮素对断奶广丰羔羊的体高、胸围、体长、体质量、平均日增重、平均日采食量和料重比无显著影响。2)断奶当日,Q200组羔羊血清谷草转氨酶(GOT)活性是CON组的1.89倍(P<0.05);断奶15 d,Q200组羔羊血清谷丙转氨酶(ALT)活性是CON组的1.02倍(P<0.05)。3)断奶当日,Q100组羔羊血清IgA浓度是CON组的1.48倍(P<0.05),而IgM和IL-1β浓度则分别降低26.97%和50.81%;断奶15d,Q200组羔羊血清IgG浓度是CON组的1.28倍,而IL-1β浓度则降低46.71%(P<0.05)。4)断奶当日,Q100组和Q200组羔羊血清CAT活性分别是CON组的1.61倍和1.82倍,断奶15 d时,分别是CON组的2.98倍和2.84倍(P<0.05);断奶15 d,Q100组羔羊血清T-SOD和GSH-Px含量分别是CON组的1.59倍和1.24倍(P<0.05),Q200组羔羊血清T-SOD和GSH-Px含量分别是CON组的1.65倍和1.32倍(P<0.05)。综上所述,槲皮素能够提高早期断奶广丰山羊羔羊的抗氧化性能和免疫力、抑制炎症反应来缓解早期断奶应激反应。
Thirty-six healthy Guangfeng goat lambs aged around 45 d with body weight(6.30±0.54)kg were selected and randomly divided into 3 groups with 12 lambs in each group.The control group was fed a basal diet(CON),and the experimental group was fed a basal diet supplemented with 100 mg/kg(Q100)or 200 mg/kg(Q200)of quercetin for 15 d.After weaning at 60 day of age,the lambs were further fed for 15 d.Body size indices of lambs were measured 15 d before weaning,on the day of weaning and 15 d after weaning.In addition,serum biochemical,immunological and antioxidant parameters were measured in lambs on the day of weaning and 15 d after weaning.The results showed that 1)the addition of quercetin to the basal diet had no significant effect on body height,chest circumference,body length,body weight,average daily gain,average daily feed intake and feed-to-weight ratio of weaned Guangfeng goat lambs.2)On the day of weaning,serum glutamate aminotransferase(GOT)activity of lambs in the Q200 group was significantly increased by 1.89 folds compared with CON group(P<0.05);at 15 d after weaning,the serum alanine aminotransferase(ALT)of lambs in the Q200 group was significantly increased by 1.02 folds compared with CON group(P<0.05).3)On the day of weaning,the serum IgA concentration of lambs in the Q100 group was significantly increased by 1.48 folds compared with CON group(P<0.05),while the IgM and IL-1βconcentrations were decreased by 26.97%and 50.81%,respectively;at 15 d after weaning,the serum IgG concentration of lambs in the Q200 group was significantly increased by 1.28 folds compared with CON group,whereas the IL-1βconcentration was decreased by 46.71%(P<0.05).4)On the day of weaning,the serum CAT activities of lambs in Q100 and Q200 groups were 1.61 and 1.82 folds higher than those in the CON group,respectively,while they were 2.98 and 2.84 folds higher than those in the CON group on 15 d after weaning(P<0.05);at 15 d after weaning,the serum T-SOD and GSH-Px contents of lambs in the Q100 group were 1.59 and 1.24 folds higher than those in the CON group,respectively(P<0.05),and the serum T-SOD and GSH-Px contents of lambs in the Q200 group were 1.65 and 1.32 folds higher than those in the CON group,respectively(P<0.05).In conclusion,quercetin can improve the antioxidant property and immunity of early weaned Guangfeng goat lambs,and inhibit the inflammatory response to alleviate the early weaning stress of Guangfeng goat lambs.
作者
王海波
谷志勇
刘远
贾浩滨
江浩筠
潘月
李文杨
赵生国
霍俊宏
占今舜
WANG Haibo;GU Zhiyong;LIU Yuan;JIA Haobin;JIANG Haoyun;PAN Yue;LI Wenyang;ZHAO Shengguo;HUO Junhong;ZHAN Jinshun(Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Science,Nanchang,Jiangxi 330200,China;College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China;Institute of Animal Husbandry and Veterinary Science,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China)
出处
《湖南农业大学学报(自然科学版)》
北大核心
2025年第1期90-97,共8页
Journal of Hunan Agricultural University(Natural Sciences)
基金
江西现代农业科研协同创新专项(JXXTCXN202203)
国家自然科学基金项目(32360857)
江西省现代农业产业技术体系建设专项(JXARS-13-肉羊岗位)。
关键词
槲皮素
广丰山羊断奶羔羊
血清生化指标
免疫
抗氧化
quercetin
Guangfeng goat weaned lambs
serum biochemical index
immunity
antioxidant
作者简介
王海波(1992-),男,甘肃会宁人,博士研究生,主要从事反刍动物营养研究,wanghaibo8815@163.com;通信作者:占今舜,博士,助理研究员,主要从事反刍动物营养与饲料研究,zhanjinshun1985@163.com。
