摘要
选用乙醇水浴加热提取法,以开口箭根状茎粉末为原料,考察料液比、乙醇体积分数、提取时间、提取温度对总皂苷得率的影响,利用正交试验优化总皂苷的提取工艺,并在此基础上,探究总皂苷提取物的抑菌活性.提取工艺优化结果表明,开口箭根状茎中总皂苷最佳提取条件为料液比1∶30(g∶mL),乙醇体积分数60%,提取温度80℃,提取时间60 min,最佳提取工艺条件下的总皂苷得率为17.46%.抑菌试验结果表明,质量浓度为20 g/L的总皂苷提取物对金黄色葡萄球菌、大肠杆菌、白色念珠菌均有抑制作用,其中对金黄色葡萄球菌的抑菌效果最佳,抑菌圈直径达19 mm.
The total saponin was isolated from Rohdea chinensis rhizome through the ethanol water bath heating extraction method.The influences of ethanol concentration,liquid to material ratio,extraction time and extraction temperature on the yield rate of the total saponin were investigated.On the basis of single factor test,the orthogonal test was used to optimize the extraction process.The optimal extraction conditions of total saponins from the rhizome of Rohdea chinensis are as follows:material-liquid ratio 1∶30(g∶mL),ethanol concentration 60%,extraction time 60 min,and extraction temperature 80℃.Under these extraction conditions,the total saponin yield of Rohdea chinensis was 17.46%.The results of the antibacterial activity test showed that the total saponins in the rhizome of the Rohdea chinensis had inhibitory effects on Escherichia coli,Staphylococcus aureus,Candida albicans,and the inhibitory activity on Staphylococcus aureus was the best,with the diameter of the inhibition circle 12 mm at a concentration of 5 g/L.
作者
郭炜
毛嘉怡
徐亮
卿笃江
成江
姚姝凤
GUO Wei;MAO Jiayi;XU Liang;QING Dujiang;CHENG Jiang;YAO Shufeng(School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;Chemical and Pharmacological Laboratory of Medicinal Resources in Wuling Mountainous Area,Jishou University,Jishou 416000,Hunan China)
出处
《吉首大学学报(自然科学版)》
2024年第6期31-36,共6页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省大学生创新创业训练计划项目(S202310531078)
吉首大学2021年引进人员资助研究项目
吉首大学校级科研项目(JDX22022)。
关键词
开口箭根状茎
总皂苷
提取工艺
抑菌活性
正交试验
rhizomes of Rohdea chinensis
total saponins
extraction process
antibacterial activity
orthogonal test
作者简介
通信作者:姚姝凤(1991-),女,山西大同人,吉首大学药学院讲师,博士,主要从事药用植物资源化学研究.E-mail:yshufly@163.com.
