摘要
干旱严重影响梨生产,梨主要砧木杜梨(Pyrus betulifolia Bunge)有抗旱特点,挖掘和鉴定其抗旱基因资源对梨耐旱育种具有重要意义。利用RNA-seq测序技术,以组培杜梨细苗经15%聚乙二醇6000干旱胁迫后0(对照)、24和48 h的叶片为材料构建mRNA文库,经测序最终获得854138996个clean reads。与对照相比,干旱胁迫24 h和48 h的差异表达基因分别有3660和2078个;与干旱胁迫24 h相比,干旱胁迫48 h的差异表达基因共有2212个。GO富集分析表明,差异表达基因主要富集在光合作用、有机环状化合物合成、信号识别、钙离子结合、氧化还原酶活性。KEGG分析表明,主要差异的代谢通路为果糖和甘露糖代谢、类黄酮生物合成、植物激素信号转导、光合固碳作用和碳代谢等。经RT-qPCR验证,RNA-seq数据可靠。
Drought seriously affects the production of pears.The main rootstock of pears,Pyrus betulifolia Bunge has drought resistance characteristics.Exploring and identifying these drought resistance gene resources is important for improving the drought resistance of pear trees.The mRNA libraries were constructed using P.betulifolia Bunge in vitro leaves treated with 15%polyethylene glycol(PEG)6000 drought stress for 0(CK),24,and 48 hours by RNA-seq sequencing technology.After sequencing,854,138,996 bp clean reads were obtained.Compared to the control,3,660 and 2,078 genes were differentially expressed at 24 and 48 hours after stress treatment,respectively.A total of 2,212 genes expressed differentially at 48 hours compared with at 24 hours after stress treatment.GO enrichment analysis showed that differentially expressed genes were mainly enriched in photosynthesis,synthesis of organic cyclic compounds,signal recognition,calcium ion binding,and oxidoreductase activity.KEGG analysis showed that the main differential metabolic pathways were fructose and mannose metabolism,flavonoid biosynthesis,plant hormone signal transduction,photosynthetic carbon fixation and carbon metabolism.After RT-qPCR validation,it was confirmed that the data of RNA seq was reliable.
作者
王金星
王中华
杨青松
阚佳亮
王宏
李晓刚
WANG Jinxing;WANG Zhonghua;YANG Qingsong;KAN Jialiang;WANG Hong;LI Xiaogang(Pomology Research Institute of Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu,210014,China)
出处
《中国南方果树》
北大核心
2025年第1期112-116,共5页
South China Fruits
基金
江苏省农业科技自主创新资金项目[CX(23)1011]
江苏省种业振兴揭榜挂帅项目(JBGS[2021]084)资助。
关键词
杜梨
干旱胁迫
转录组
差异表达基因
Pyrus betulifolia Bunge
drought stress
transcriptome
differentially expressed genes
作者简介
第一作者:王金星,男,助理研究员。E-mail:xing521_zi@163.com;通信作者:李晓刚,男,研究员。E-mail:xiaogangli@aliyun.com。
