摘要
目的探究PI3K/AKT/NF-κB通路调控胶质母细胞瘤替莫唑胺耐药机制。方法选取2018年8月至2023年8月福建省泉州市第一医院收治的78例胶质母细胞瘤患者作为研究对象,收集肿瘤组织及肿瘤周围水肿带脑组织,比较观察组织、细胞中PI3K、AKT、NF-κB的表达水平及分析细胞中PI3K、AKT、NF-κB的调控关系和U251/TMZ增殖情况。结果胶质母细胞瘤组织的PI3K、AKT、NF-κB表达水平分别是(1.88±0.21)、(1.85±0.25)、(1.82±0.23)高于肿瘤周围水肿带脑组织的(0.82±0.13)、(0.89±0.16)、(0.84±0.14)(P<0.05)。U251/TMZ细胞中PI3K、AKT、NF-κB的表达水平分别是(1.87±0.24)、(1.83±0.22)、(1.83±0.21)高于U251细胞的(0.84±0.12)、(0.81±0.15)、(0.83±0.16)(P<0.05)。敲低PI3K,AKT、NF-κB表达明显下降,且通过对AKT、NF-κB的抑制,PI3K的表达水平也明显下降(P<0.05),且敲低PI3K对照组、抑制AKT、NF-κB组的U251/TMZ细胞增殖活性均显著性降低(P<0.05)。结论PI3K/AKT/NF-κB通路可调控胶质母细胞瘤增殖,改变胶质瘤细胞替莫唑胺耐药性,具有重要的研究价值。
Objective To explore the mechanism of PI3K/AKT/NF-κBpathway regulating temozolomide resistance in glioblastoma.Methods Seventy-eight patients with glioblastoma admitted to the First Hospital of Quanzhou City,Fujian Province from August 2018 to August 2023 were selected as the research subjects.Tumor tissue and peritumoral edema zone brain tissue were collected to compare and observe the expression levels of PI3K,AKT,NF-κB in tissues and cells,as well as analyze the regulatory relationship of PI3K,AKT,NF-κB in cells and the proliferation of U251/TMZ.Results The expression levels of PI3K,AKT,and NF-κB in glioma tissue were higher(1.88±0.21),(1.85±0.25),and(1.82±0.23)than those in adjacent tissues(0.82±0.13),(0.89±0.16),and(0.84±0.14),respectively(P<0.05).The expression levels of PI3K,AKT,and NF-κB in U251/TMZ cells were(1.87±0.24),(1.83±0.22),and(1.83±0.21),respectively,higher than those in U251 cells(0.84±0.12),(0.81±0.15),and(0.83±0.16)(P<0.05).Knocking down PI3K significantly reduced the expression of AKT and NF-κB,and through inhibition of AKT and NF-κB,the expression level of PI3K also decreased significantly(P<0.05).In addition,the U251/TMZ cell proliferation activity in the PI3K knockdown control group and the AKT and NF-κB inhibition group was significantly reduced(P<0.05).Conclusions The PI3K/AKT/NF-κB pathway can regulate the proliferation of glioblastoma and alter the resistance of glioma cells to temozolomide,which has important research value.
作者
王熹照
WANG Xizhao(Department of Neurosurgery,The First Hospital of Quanzhou,Quanzhou,Fujian 362000,China)
出处
《中国医药指南》
2025年第6期39-41,共3页
Guide of China Medicine
