摘要
WIP蛋白属于C2H2锌指蛋白的A1d亚组,目前研究发现CsWIP1在黄瓜中参与性别决定,但WIP基因在黄瓜生长发育过程中仍存在未知的功能。在黄瓜、甜瓜、西瓜、冬瓜、葫芦和美洲南瓜中鉴定出40个WIP基因家族成员。利用在线网站对葫芦科WIP蛋白进行理化性质分析,通过生物信息学进行染色体定位、基因结构等分析。对黄瓜WIP基因的顺式元件进一步分析表明,WIP家族基因具有空间表达、植物激素、光响应元件等顺式作用元件。黄瓜不同组织的实时荧光定量PCR分析发现,CsaV3_1G014730和CsaV3_4G031370可能在黄瓜的顶芽发育中发挥重要作用;CsaV3_4G024150(CsWIP1)、CsaV3_3G039140、CsaV3_7G031140和CsaV3_5G023030则可能参与雌花、雄花、传输通道以及雄配子的生长发育,推测WIP基因可能在黄瓜传输通道发育、叶脉模式调控等过程中起着重要的作用。
The WIP transcription factor family belongs to the A1d subgroup of C2H2 zinc finger protein.At present,CsWIP1 is involved in sex determination in cucumber,but the function of WIP gene in cucumber growth and development is still unknown.This study identified 40 members of the WIP gene family in cucumber,melon,watermelon,wax gourd,bottle gourd,and zucchini.Using online websites to analyze the physicochemical properties of the WIP proteins in the Cucurbitaceae family,and conducting chromosomal localization,gene structure,and other analyses through bioinformatics.The analysis of the cis acting elements of cucumber WIP genes indicates that the WIP family genes have cis acting elements such as spatial expression,plant hormones,and photoresponse elements.Real time fluorescence quantitative PCR analysis of different tissues in cucumber revealed that CsaV3_1G014730 and CsaV3_4G031370 may play important roles in the shoot apex of cucumber;CsaV3_4G024150(CsWIP1),CsaV3_3G039140,CsaV3_7G031140,and CsaV3_5G023030 may be involved in the growth and development of female and male flowers,transmitting tract,and male gametes,suggesting that WIP gene may play an important role in the development of transport channel and regulation of leaf vein pattern in cucumber.
作者
韩瑞月
刘晓林
辛同旭
李森
杨学勇
孙进京
HAN Ruiyue;LIU Xiaolin;XIN Tongxu;LI Sen;YANG Xueyong;SUN Jinjing(College of Horticulture,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,State Key Laboratory of Vegetable Biobreeding,Beijing 100081,China)
出处
《中国蔬菜》
北大核心
2025年第2期44-55,共12页
China Vegetables
基金
中国农业科学院科技创新工程项目(CAAS-ASTIP)。
关键词
黄瓜
WIP基因家族
转录因子
生物信息学分析
组织表达模式
cucumber
WIP gene family
transcription factor
bioinformatics analysis
tissue expression pattern
作者简介
韩瑞月,女,硕士研究生,专业方向:农艺与种业,E-mail:3491721912@qq.com;通信作者:杨学勇,男,博士,研究员,专业方向:蔬菜发育生物学,E-mail:yangxueyong@caas.cn;通信作者:孙进京,女,博士,研究员,专业方向:黄瓜单性花发育,E-mail:sunjinjing@caas.cn。
