摘要
探讨红光和蓝光补光影响三叶青Tetrastigma hemsleyanum光合作用及其调控机制,为筛选三叶青合适的光照条件提供参考依据。以三叶青种苗为试材,以白光为对照,测定在红、蓝光补光的三叶青叶片叶绿素含量及其净光合速率变化,并利用Illumina高通量测序技术进行转录组测序和生物信息学分析。结果表明,红、蓝光补光的三叶青叶片叶绿素含量和净光合速率均高于白光,且蓝光显著高于其他处理组;组装共获得73 598条Unigenes,其中注释差异表达Unigenes(DEGs)1831条,在三叶青光合作用相关通路中共鉴别出30个DEGs,包含20种酶。红、蓝光补光可以增加三叶青叶绿素含量和净光合速率,且蓝光效果最佳,初步揭示红、蓝光调控三叶青光合作用的分子机制,为改进三叶青的野生驯化与人工栽培奠定基础。
In this study,the effects of red and blue light supplementation on photosynthesis and its regulatory mechanism of Tetrastigma hemsleyanum were investigated,providing a reference for selecting suitable light conditions for T.hemsleyanum.Taking the seedlings of T.hemsleyanum as experiment materials and the white light as the control,the changes of chlorophyll content and net photosynthetic rate of leaves in red and blue light supplementation were measured,and transcriptome sequencing and bioinformatics analysis were performed by Illumina high-throughput sequencing technology.The results showed that the chlorophyll contents and net photosynthetic rates of T.hemsleyanum leaves in red and blue light supplementation were higher than those in white light,and the blue light was significantly higher than those of other treatment groups.A total of 73598 unigenes were obtained through assembly,among which 1831 differentially expressed unigenes(DEGs)were annotated.A total of 30 DEGs,including 20 enzymes,were identified in the photosynthesis-related pathways of T.hemsleyanum.Red and blue light supplementation could increase the chlorophyll content and net photosynthetic rate of T.hemsleyanum,and the blue light effect had the best effect,which preliminarily revealed the molecular mechanism of red and blue light regulating the photosynthesis of T.hemsleyanum,and laid a foundation for improving the wild domestication and artificial cultivation of T.hemsleyanum.
作者
洪玉燕
杨楠
潘梦婷
林绵发
许文
徐惠龙
范世明
HONG Yu-yan;YANG Nan;PAN Meng-ting;LIN Mian-fa;XU Wen;XU Hui-long;FAN Shi-ming(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian China;Science and Technology Innovation and Transformation Center,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian China)
出处
《亚热带植物科学》
2024年第5期408-415,共8页
Subtropical Plant Science
基金
福建省自然科学基金项目(2016N0015、2018N0012、2021Y4015、2021S2015、2022N5018)
福建中医药大学校管课题(X2021013-重点)
厦门市科技特派员专项(20222004)
国家中医药管理局2023年中央财政转移支付地方项目《中药资源保障能力提升项目》
关键词
三叶青
转录组
红蓝光补光
光合作用
差异表达基因
Tetrastigma hemsleyanum
transcriptome
red and blue light supplementation
photosynthesis
differentially expressed genes
作者简介
通信作者:徐惠龙。E-mail:hlongxu@163.com
