摘要
线粒体是真核细胞的细胞器,是细胞能量代谢、氧化应激、热量生成以及信号转导的重要场所。线粒体移植(mitochondrial transplantation,MT)是目前治疗线粒体功能障碍及抗衰老研究最为前沿的技术手段。本研究将牦牛(Bos grunniens)、普通牛(Bos taurus)和马(Equus caballus)的线粒体移植至小鼠(Mus musculus),旨在探究异种线粒体移植的可行性及作用效果。研究结果显示,通过共聚焦成像、数字PCR及DNA测序检测到牦牛、普通牛和马的线粒体能够移植至小鼠体内,并在小鼠广泛的组织、器官中至少可维持14 d。MT小鼠产生了积极的生物学效应,包括提高ATP含量和线粒体DNA拷贝数(P<0.05),其效应可维持到14 d,并在第7 d达到最大值。MT小鼠活性氧(ROS)水平表现为初期(注射2 h)显著上升(P<0.05),在第7 d和第14 d逐渐下降至接近对照组水平(P>0.05)。研究结果支持了异种线粒体移植的有效性,并表明其效果可以维持至14 d。本研究为未来的临床应用提供了科学依据。
Mitochondria are organelles in eukaryotic cells that play a crucial role in cellular energy metabolism,oxidative stress,heat production,and signal transduction.Mitochondrial transplantation(MT)is currently one of the most advanced techniques for treating mitochondrial dysfunction and anti-aging research.This study aimed to explore the feasibility and effectiveness of xenogeneic MT by transplanting mitochondria from yak(Bos grunniens),domestic cattle(Bos taurus),and horse(Equus caballus)into mice(Mus musculus).The results demonstrated that mitochondria from yak,domestic cattle,and horse could be successfully transplanted into mice and maintained in various tissues and organs of the mice for at least 14 days,as confirmed by confocal imaging,digital PCR,and DNA sequencing.MT mice exhibited positive biological effects,including increased ATP content and mitochondrial DNA copy number(P<0.05),with the maximum effect observed on day 7,which was sustained until day 14.Reactive oxygen species(ROS)levels in MT mice significantly increased at 2 hours post-injection(P<0.05),then gradually decreased towards baseline levels by day 7 and day 14(P>0.05).These findings support the effectiveness of xenogeneic MT and suggest that the effects can be maintained for up to 14 days.This study provides scientific evidence for future clinical applications.
作者
杨圳
李文鹏
牛添
薛慧雯
赵思喜
赵兴波
YANG Zhen;LI Wen-Peng;NIU Tian;XUE Hui-Wen;ZHAO Si-Xi;ZHAO Xing-Bo(State Key Laboratory of Animal Biotech Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;College of Basic Medicine,Capital Medical University,Beijing 100069,China)
出处
《中国生物化学与分子生物学报》
北大核心
2025年第2期273-283,共11页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家重点研发计划(No.2021YFF1000701)资助。
关键词
线粒体移植
异种线粒体
线粒体DNA拷贝数
三磷酸腺苷
活性氧
mitochondrial transplantation(MT)
xenomitochondria
mtDNA copy number
adenosine triphosphate(ATP)
reactive oxygen species(ROS)
作者简介
通讯作者:赵兴波,Tel:010-62733417,E-mail:zhxbcau@163.com。
