摘要
旨在探究PLA2G4A基因对坝上长尾鸡腿肌肌肉组织中肌苷酸(IMP)的调控水平,为进一步探究坝上长尾鸡PLA2G4A基因的生物学功能奠定基础。以选取270日龄的坝上长尾鸡腿为试验材料,将坝上长尾鸡腿肌组织以肌苷酸含量高低进行分组,每组各5个样本,从腿肌组织中提取总RNA,对其进行转录组测序分析,并进行KEGG富集分析GO功能注释和蛋白互作网络分析;利用RT-qPCR技术,分析差异基因在坝上长尾鸡腿肌组织中的mRNA表达水平;从测序结果中筛选参与甘油磷酸酯代谢通路的基因PLA2G4A。KEGG富集分析结果表明,与肌苷酸合成相关的信号通路包括甘油磷酸酯代谢、醚脂代谢、花生四烯酸代谢等。GO功能注释表明,PLA2G4A基因参与11个生物学过程,包括甘油磷酸酯代谢,醚脂代谢、花生四烯酸代谢、亚油酸代谢等。蛋白互作发现,PLA2G4A基因与花生四烯酸代谢和甘油磷酸酯代谢过程中的重要调控酶共享功能。RT-qPCR结果显示,坝上长尾鸡腿肌组织中PLA2G4A基因的表达量在样本中存在显著性差异。综上所述,PLA2G4A基因可能是影响鸡肉IMP沉积的主效基因。
In order to investigate the regulatory level of PLA2G4A gene on inosine monophosphate(IMP)in the leg muscle tissue of Bashang Long-tailed chicken,and further explore the biological function of PLA2G4A gene in Bashang Long-tailed chicken.The leg muscle tissue of 270-day-old Bashang Long-tailed chicken was categorized based on the inosine monophosphate content,with five samples allocated to each group.Transcriptome sequencing a-nalysis was conducted on the muscle tissue,followed by KEGG enrichment analysis,GO function annotation and pro-tein interaction network analysis.RT-qPCR was used to analyze the mRNA expression level gene in the leg muscle tissue of Bashang Long-tailed chicken.KEGG enrichment analysis showed that the signal pathways related to IMP synthesis included glycerophospholipid metabolism,etherlipid metabolism and arachidonic acid metabolism.GO functional annotation showed that the PLA2G4A gene was involved in 11 biological processes,including glycerophos-phate metabolism,etherlipid metabolism,arachidonic acid metabolism and linoleic acid metabolism.Protein interac-tion revealed that the PLA2G4A gene shared important regulatory enzyme functions in arachidonic acid metabolism and glycerophosphate metabolism.The Real-time Fluorescence Quantitative PCR results showed that there was a sig-nificant difference in the expression level of the PLA2G4A gene in the leg muscle tissue of Bashang Long-tailed chicken in the sample.In summary,the PLA2G4A gene may be the main gene affecting IMP deposition in chicken.
作者
何玲钰
乔贤
王新越
李祥龙
HE Lingyu;QIAO Xian;WANG Xinyue;LI Xianglong(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066000,China;Hebei Provincial Key Laboratory of Characteristic Animal Germplasm Resources Mining and Innovation,Qinhuangdao 066000,China)
出处
《华北农学报》
CSCD
北大核心
2024年第S1期329-336,共8页
Acta Agriculturae Boreali-Sinica
基金
河北省鸡现代种业科技创新团队(21326303D)。
作者简介
何玲钰(1999-),女,河北石家庄人,在读硕士,主要从事动物遗传育种研究;通信作者:李祥龙(1963-),男,河北保定人,教授,博士,博士生导师,主要从事动物遗传育种研究。
