摘要
目的利用体内和体外实验探讨金磁纳米复合材料对胃癌的抑制作用。方法将胃癌细胞分为Ctrl组(不添加任何纳米材料)和实验组(金磁纳米材料处理)。采用噻唑蓝(MTT)法检测细胞增殖活力;细胞周期检测试剂盒检测细胞G1期、G2期、S期的细胞比例;Western blot检测细胞中细胞增殖核相关抗原(Ki67)、细胞周期蛋白D1(CyclinD1)、切割模式的半胱天冬酶3(cleaved-caspase 3)、B细胞淋巴瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)、血管内皮生长因子A(VEGF-A)、基质金属蛋白酶(MMP)-2、MMP-9、神经钙黏蛋白(N-cadherin)、上皮钙黏蛋白(E-cadherin)的表达水平。将30只成年C57BL/6小鼠均接种胃癌细胞,待肿瘤体积达100 mm3后随机分为空白组(尾静脉注射100μl PBS)、观察组(尾静脉注射100μl 2 mg/ml的光热磁纳米材料)及对照组(尾静脉注射100μl 2 mg/ml的光热碳纳米材料),每组10只。采用近红外激光照射小鼠的整个肿瘤区域,30 d后测量3组小鼠肿瘤体积和质量的变化。结果实验组和Ctrl组SNU-16及KWS-1细胞培养7 d的细胞增殖活力均高于同组同类型细胞培养3 d,实验组SNU-16和KWS-1细胞培养3 d及7 d的细胞增殖活力、S期细胞比例、Ki67、CyclinD1、Bcl-2、VEGF-A、MMP-2、MMP-9、N-cadherin表达水平均低于Ctrl组,G1期细胞比例、Bax、cleaved-Caspase 3及E-cadherin表达水平均高于Ctrl组(P<0.05)。空白组、对照组和观察组小鼠第30 d肿瘤质量和肿瘤体积均依次下调(P<0.05)。结论金磁纳米复合材料化疗治疗对肿瘤细胞的杀伤率更高,肿瘤体积更小。金磁纳米复合材料对胃癌细胞具有抑制作用。
Objective To investigate the inhibitory effect of gold-coated magnetic nanocomposites on gastric cancer by in vivo and in vitro experiments.Methods Gastric cancer cells were divided into Ctrl group(without adding any nanomaterials)and experimental group(gold magnetic nanomaterials treatment).Methyl thi-azolyl tetrazolium(MTT)assay was used to detect cell proliferation activity;Cell cycle detection kit was used to detect the proportion of cells in G1,G2 and S phases;Western blot was used to detect the expression levels of nuclear associated antigen(Ki67),Cyclin D1,cleaved cysteinyl aspartate specific proteinase 3(cleaved-caspase 3),B cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),vascular endothelial growth factor A(VEGF-A),matrix metalloproteinase(MMP)-2,MMP-9,Neural-cadherin(N-cadherin)and Epithelial-cadherin(E-cadherin).Thirty adult C57BL/6 mice were inoculated with gastric cancer cells.When the tumor volume reached 100 mm~3,the mice were randomly divided into blank group(100μl PBS,tail vein injection),observation group(100μl 2 mg/ml photothermal magnetic nanomaterials,tail vein injection)and control group(100μl 2 mg/ml photothermal carbon nanomaterials,tail vein injection),with 10 mice in each group.The whole tumor area of the mice was irradiated by near-infrared laser.After 30 days,the changes of tumor volume and mass in three groups were measured and compared.Results The proliferation activity of SNU-16 and KWS-1 cells in experimental group and Ctrl group at 7 days was higher than that of the same type cells at 3 days;The cell proliferation activity at 3 and 7 days,S phase cell proportion,Ki67,CyclinD1,Bcl-2,VEGF-A,MMP-2,MMP-9 and N-cadherin expression levels of SNU-16 and KWS-1 cells in experimental group were lower than those in Ctrl group.The proportion of cells in G1 phase and the expression levels of Bax,cleaved-Caspase 3 and E-cadherin were significantly higher than those in Ctrl group(P<0.05).The tumor volume and mass of mice in blank group,control group and observation group were successively down-regulated(P<0.05).Conclusion Gold-coated magnetic nanocomposites has higher killing rate and smaller tumor volume.Gold-coated magnetic nanocomposites can inhibit gastric cancer cells.
作者
吴江
庞澜
冷静
马秀英
吴涛
Wu Jiang;Pang Lan;Leng Jing;Ma Xiuying;Wu Tao(Endoscopic Diagnosis and Treatment Center,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China;不详)
出处
《临床内科杂志》
2024年第12期848-851,共4页
Journal of Clinical Internal Medicine
基金
新疆维吾尔自治区自然科学基金资助项目(2021D01C373)。
关键词
胃癌
肿瘤体积
细胞增殖
细胞周期
金磁纳米复合材料
Gastric cancer
Tumor volume
Cell proliferation
Cell cycle
Gold-coated magnetic nanocomposite
作者简介
通讯作者:吴涛,E-mail:1852623400@qq.com。
