摘要
为了解信阳地区鹅源大肠杆菌的系统进化分群、毒力基因携带情况和耐药情况,试验从信阳地区部分养殖户送检的46只疑似大肠杆菌感染病鹅的肝脏、脾脏等组织中进行大肠杆菌的分离、鉴定,并对分离菌株进行系统进化分群、毒力基因检测、耐药性分析、耐药基因检测。结果表明:共分离到23株大肠杆菌,分别命名为GE1~GE23。23株大肠杆菌在4种系统进化群中均有一定比例的分布,其中A群有3株(GE11、GE12、GE19,占比为13.04%),B1群有3株(GE4、GE15、GE23,占比为13.04%),B2群有9株(GE1、GE2、GE8、GE10、GE14、GE16、GE17、GE20、GE21,占比为39.13%),D群有8株(GE3、GE5、GE6、GE7、GE9、GE13、GE18、GE22,占比为34.78%)。在毒力基因检测中,23株大肠杆菌marA、fimC、fyuA、iroN、iss、hlyF、ompT、iutA和irp2基因的检出率分别为100%、95.65%、52.17%、47.83%、43.48%、39.13%、34.78%、30.43%和17.39%。23株分离菌对阿莫西林/克拉维酸钾的耐药率最高,为65.22%;然后依次为磺胺甲口恶唑/甲氧苄啶、强力霉素、氟苯尼考、多黏毒素B、头孢噻呋和左氧氟沙星、环丙沙星,耐药率分别为56.52%、52.17%、47.83%、34.78%、17.39%和17.39%、8.70%。23株分离菌对亚胺培南的敏感率最高,为100%;然后依次为丁胺卡那、链霉素、新霉素、环丙沙星和左氧氟沙星、头孢噻呋、氟苯尼考、多黏菌素B、强力霉素、磺胺甲口恶唑/甲氧苄啶,敏感率分别为82.61%、82.61%、47.83%、47.83%和47.83%、34.78%、26.09%、21.74%、17.39%、8.70%。23株分离菌中有12株表现为多重耐药,其中6,7耐菌株各有1株,占比为4.35%;4,5耐菌株各有3株,占比为13.04%;3耐菌株有4株,占比为17.39%。在耐药基因检测中,tetA基因的检出率最高,为82.61%;然后依次为folR、sul2、aadA、bla_(TEM)、bla_(CTX-M)、aphA1、clmA、sul3、sul1、aphA2,检出率分别为73.91%、65.22%、56.32%、52.17%、47.83%、47.83%、43.48%、26.09%、13.04%和13.04%。说明信阳地区鹅源大肠杆菌B2群和D群占比较大,携带多种毒力基因和耐药基因,且多表现为多重耐药。
In order to understand the phylogenetic clustering,virulence gene carrying and drug resistance of goose-derived E.coli in Xinyang area,in the experiment,isolation and identification of E.coli were carried out from the liver,spleen and other tissues of 46 suspected E.coli infected geese sent by some farmers in Xinyang area;the isolated strains were carried out for systematical clustering,virulence gene detection,drug resistance analysis,and drug resistance gene detection.The results showed that a total of 23 strains of E.coli were isolated and named GE1-GE23.The 23 strains of E.coli were distributed in a certain proportion of the four phylogenetic groups,including 3 strains in group A(GE11,GE12,GE19,accounting for 13.04%),3 strains in group B1(GE4,GE15,GE23,accounting for 13.04%),9 strains in group B2(GE1,GE2,GE8,GE10,GE14,GE16,GE17,GE20,GE21,accounting for 39.13%),and 8 strains in group D(GE3,GE5,GE6,GE7,GE9,GE13,GE18,GE22,accounting for 34.78%).The detection rates of marA,fimC,fyuA,iroN,iss,hlyF,ompT,iutA and irp2 genes in 23 strains of Escherichia coli were 100%,95.65%,52.17%,47.83%,43.48%,39.13%,34.78%,30.43%and 17.39%,respectively.The 23 isolates had the highest resistance rate to amoxicillin/clavulanate potassium,which was 65.22%.Then sulfamethoxazole/trimethoprim,doxycycline,florfenicol,polymyxoxin B,ceftiofur,levofloxacin and ciprofloxacin were followed,and the drug resistance rates were 56.52%,52.17%,47.83%,34.78%,17.39%,17.39%and 8.70%,respectively.The 23 isolates had the highest susceptibility rate to imipenem,which was 100%.Then amicana,streptomycin,neomycin,ciprofloxacin and levofloxacin,cefotaxifur,florfenicol,polymyxin B,doxycycline,sulfamethoxazole/trimethoprim were followed,and the sensitivity rates were 82.61%,82.61%,47.83%,47.83%and 47.83%,34.78%,26.09%,21.74%,17.39%and 8.70%,respectively.Among the 23 isolates,12 strains showed multi-drug resistance,of which one strain was 6-resistant and one was 7-resistant,each accounting for 4.35%.There were three 4-resistant and 5-resistant strains,each accounting for 13.04%;there were four 3-resistant strains,accounting for 17.39%.In the detection of drug resistance genes,the detection rate of tetA gene was the highest,which was 82.61%;then followed by folR,sul2,aadA,bla_(TEM),bla_(CTX-M),aphA1,clmA,sul3,sul1 and aphA2,which were detected at 73.91%,65.22%,56.32%,52.17%,47.83%,47.83%,43.48%,26.09%,13.04%and 13.04%,respectively.The results indicated that the B2 and D groups of E.coli from goose in Xinyang area accounted for a large proportion,carrying a variety of virulence genes and drug resistance genes,and most of them showed multi-drug resistance.
作者
李迎晓
方燕子
武娴
曲哲会
焦凤超
LI Yingxiao;FANG Yanzi;WU Xian;QU Zhehui;JIAO Fengchao(Xinyang Agriculture and Forestry University,Xinyang 464000,China;Henan Provincial Engineering Technology Research Center for Waterfowl Resources Development and Utilization and Epidemic Disease Prevention and Control,Xinyang 464000,China)
出处
《黑龙江畜牧兽医》
北大核心
2025年第1期82-93,共12页
Heilongjiang Animal Science And veterinary Medicine
基金
信阳农林学院科技创新团队项目(XNKJTD-014)
信阳农林学院科技服务团队项目
信阳农林学院青年教师基金项目(QN2022005)。
关键词
鹅
大肠杆菌
系统进化分群
毒力基因
耐药性
耐药基因
goose
Escherichia coli
phylogenetic clustering
virulence gene
drug resistance
drug resistance gene
作者简介
李迎晓(1981-),女,副教授,硕士,研究方向为兽医微生物与免疫学,liyingxiao81@163.com;通信作者:焦凤超(1979-),男,副教授,硕士,研究方向为兽医微生物与免疫学,fengchaoajiao@163.com.
