摘要
目的 建立血液中阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓等5种苯二氮卓类违禁药品的表面增强拉曼光谱(SERS)快速检测方法。方法 采用乙腈作为提取剂对血液中的阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓进行提取,添加PSA和C18混合物进行优化,并使用SERS技术进行检测。结果 实验表明,阿普唑仑、硝基安定、替马西泮、地西泮、氯氮卓均出现了相应的拉曼特征信号峰,这些特征峰可以作为定性的判别依据。此外,这5种苯二氮卓类药物在血液中的检出限在0.5~1μg/mL之间。结论 本文应用表面增强拉曼技术,通过添加PSA和C18混合物进行前处理优化获得了良好的拉曼增强信号,该方法操作简单,分析速度快,适用于血液样品中药品的快速检测。
Objective To establish a rapid surface-enhanced Raman spectroscopy(SERS)method for detection of five banned benzodiazepines in blood,including alprazolam,nitrovalam,Temazepam,diazepam and cloazepine.Methods Ethanol was used as extraction agent to extract nitrodiazepam,nitrazepam,diazepam and clonazepam from blood,a mixture of PSA and C18 was added for optimization,and SERS technology was used for detection.Results Experiments showed that nitalprazolam,nitrodiazepam,Temazepam,diazepam and chlorazepine all had corresponding Raman characteristic signal peaks,which could be used as the basis for qualitative discrimination.The detection limits of these benzodiazepines in blood were between 0.5 and 1μg/mL.Conclusion This method is simple to operate and has a fast analysis speed.It is suitable for the rapid detection of drugs in blood samples.
作者
沈靓
诸仁祥
陈芳
施碧云
钟武魁
郭郡郡
Shen Liang;Zhu Renxiang;Chen Fang;Shi Biyun;Zhong Wukui;Guo Junjun(Keqiao District Branch of Shaoxing Public Security Bureau,Shaoxing,Zhejiang 312030;Shaoxing University of Arts and Sciences,Shaoxing,Zhejiang 312000;Institute of Judicial Appraisal Science,Shanghai 200063,China;Shaoxing Public Security Bureau,Shaoxing,Zhejiang 312000)
出处
《中国法医学杂志》
CSCD
2024年第6期706-709,716,共5页
Chinese Journal of Forensic Medicine
作者简介
共同第一作者:沈靓,男,硕士,警务技术副高级任职资格,主要从事法医学鉴定和研究工作,E-mail:xiaoqiao2006@139.com;共同第一作者:诸仁祥,男,学士,主检法医师,主要从事法医学鉴定工作,E-mail:339384589@qq.com;共同第一作者:陈芳,女,主检法医师,主要从事法医学鉴定,E-mail:chenf@ssfid.cn。
