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连作对菠菜根际土壤微生物群落的影响

Effect of continuous cropping on rhizosphere soil microbial communities of spinach
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摘要 【目的】探究菠菜连作下根际土壤理化性质和微生物群落的变化。【方法】对菠菜种植1年(1Y)和连续种植2年(2Y)根际土壤的理化性质进行测定,同时采用PacBio全长多样性测序分析根际土壤中的微生物群落,比较2Y和1Y土壤中细菌和真菌微生物群落的变化,同时利用冗余分析法研究土壤理化性质和微生物群落的关系。【结果】连作后土壤理化性质发生显著变化(P<0.05),2Y土壤pH值和全氮、全磷、有效磷、铵态氮、有机质、微生物量碳含量显著高于1Y土壤(P<0.05),电导率和全钾、速效钾、硝态氮含量显著低于1Y土壤(P<0.05)。冗余分析表明,全钾是影响菠菜连作下根际土壤微生物群落的主要因子。2Y土壤中微生物群落的Chao1指数、Richness指数、Shannon指数、ACE指数均高于1Y土壤,与碳、氮循环,纤维素降解,农药和塑料降解以及镰刀菌属(Fusarium)相关的病原菌含量在2Y土壤中显著增加(P<0.05),而具有拮抗活性的溶杆菌属(Lysobacter)生防菌含量显著降低(P<0.05)。【结论】连续2年种植下的菠菜根际土壤,理化性质发生显著变化,病原菌含量增加、生防菌含量减少。 【Objective】To explore the changes in rhizosphere soil physicochemical properties and mirobial community under continuous cropping of spinach.【Methods】The physicochemical properties of the rhizosphere soil of spinach planted for 1 year(1Y)and continuously planted for 2 years(2Y)were measured,and the microbial community in the rhizosphere soil was analyzed using PacBio full-length diversity sequencing.The changes in bacterial and fungal microbial communities in the 2Y and 1Y soils were compared,and the relationship between soil physicochemical properties and microbial communities was studied using redundancy analysis.【Results】Highly significant changes in soil physicochemical properties after continuous cropping(P<0.05),and the pH value and the contents of total nitrogen,total phosphorus,available phosphorus,ammonium nitrogen,organic matter,and microbial biomass carbon were significantly higher in the 2Y soil than in the 1Y soil(P<0.05),the electrical conductivity and the contents of total potassium,available potassium,and nitrate nitrogen were significantly lower than those in the 1Y soil(P<0.05).The redundancy analysis showed that the total potassium was the main factor influencing the rhizosphere soil microbial communities under spinach continuous cropping.The microbial community of the 2Y soil Chao1 index,Richness index,Shannon index,and ACE index were higher than those of 1Y soil.Among which pathogen related to carbon and nitrogen cycle,cellulose degradation,pesticides and plastic degradation and Fusarium associated pathogen content increased significantly in 2Y soil(P<0.05),while the content of Lysobacter biocontrol bacteria with antagonistic activity was significantly decreased(P<0.05).【Conclusion】The rhizosphere soil of spinach under 2Y continuous planting showed significant changes in physicochemical properties,with an increase of pathogen content and a decrease of biocontrol bacteria content.
作者 张佳 李正男 张磊 廉勇 付崇毅 王永 孙平平 ZHANG Jia;LI Zhengnan;ZHANG Lei;LIAN Yong;FU Chongyi;WANG Yong;SUN Pingping(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China;Institute of Vegetables and Flowers,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)
出处 《北方农业学报》 2024年第5期95-107,共13页 Journal of Northern Agriculture
基金 内蒙古自治区科技重大专项(2021ZD0001) 内蒙古自治区高等学校科学研究项目(NJZY21458) 内蒙古自治区高等学校青年科技英才项目(NJYT23079) 内蒙古农业大学高层次人才科研启动金项目(NDYB2019-1)。
关键词 菠菜 连作 根际土壤 理化性质 微生物群落 Spinach Continuous cropping Rhizosphere soil Physicochemical property Microbial community
作者简介 张佳(1999-),女,硕士研究生,研究方向为园艺作物病原生物学及病害综合防控;通信作者:孙平平(1985-),女,副教授,博士,主要从事作物病原分离鉴定病害绿色防控方面的研究工作。
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