摘要
目的 :研究白细胞介素 - 1β(IL - 1β)抗体预防小鼠术后腹腔粘连的能力。 方法 :以BALB/c小鼠为动物模型 ,经过同一标准的腹膜损伤手术后 ,30只BALB/c小鼠随机分为两组 :IL - 1β抗体治疗组 ,生理盐水对照组 ,分别在关腹前腹腔内灌注IL -1β抗体及生理盐水。术后 2 0d将小鼠处死 ,观察腹腔内粘连的情况 ,通过对粘连的程度、位置以及粘连类型的综合评价得出粘连分数。结果 :IL - 1β抗体治疗组的平均粘连分数 (1.2 2± 0 .35 )明显低于对照组 (2 .31± 0 .5 7) (P <0 .0 1) ,2～ 3级粘连发生率(2 2 .6 % )比对照组 (78.3% )亦有明显减低 (P <0 .0 1) ,无 1例小鼠出现腹壁切口疝。结论 :IL -
Objective:To assess the ability of interleukin-1 beta (IL-1β) antibody to prevent postoperative abdominal adhesion formation in mice.Methods:30 BALB/c mice were randomly divided into two groups: anti-IL-1β group ( n =15) and control group ( n =15). After an identical peritoneal injury operation, mice were treated intraperitoneally with either IL-1β antibody or normal saline at the time of abdominal closure. The mice were killed 20 days after operation , the abdominal incisions and the development of intraabdominal adhesions were observed. Adhesion scores were obtained based on an overall assessment of the adhesion extent, position and types.Results:The adhesion scores (1.22± 0.35 ) of anti-IL-1β group were significantly lower than that of control group (2.31±0.57). The occurrence rate of grade 2-3 adhesions in anti-IL-1β group was 22.6%, significantly lower than that of control group (78.3%). No incisional hernia was observed in abdominal wall in all cases.Conclusion:Intraperitoneal administration of IL-1β antibody might play important roles in inhibiting postoperative adhesion formation.
出处
《西北国防医学杂志》
CAS
2002年第6期429-430,共2页
Medical Journal of National Defending Forces in Northwest China
基金
全军医药卫生科研基金资助项目 (98M10 9)
