摘要
藻红蛋白(PE)具有特征性吸收光谱和荧光光谱,是一种新颖的荧光分子探针.经反复探索研究建立了盐析、离子交换层析、羟基磷灰石吸附层析和凝胶过滤层析的分离纯化方案,从珊瑚藻中分离纯化出R-藻红蛋白(R-PE),其纯度[D(565nm)/D(280nm)]高达11.
The phycoerythrin (PE) was a kind of fluorescence molecular probe with the characteristics of absorption spectra and fluorescence spectra. The successive procedures of fraction precipitation of ammonium sulfate, ion exchange, modified hydrorylapatite and gel filtration chromatography were set up. A kind of Rphycoerythrin(RPE) from Corallina officinalis was separated and purified, the purity of RPE reached 11.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2002年第4期495-499,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省科技厅重大资助项目(2000H004和2001H127).
