摘要
本文研究了莲房原花青素(LSPCs)对极低频电磁场(ELF-EMF)致雄性小鼠生殖系统损伤的预防作用。选用50只雄性ICR小鼠,随机分为对照组,ELF-EMF组和ELF-EMF+30、60、90 mg/kg LSPCs预处理组。LSPCs连续灌胃75 d,灌胃15 d后开始连续辐射60 d。ELF-EMF辐射处理后将小鼠处死,取出附睾、睾丸并称重,求睾体比,检测附睾中精子活率和精子畸形率,测定睾丸组织超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)含量,观察睾丸组织形态学变化。结果表明ELF-EMF辐射处理后小鼠睾丸组织形态发生严重改变,睾体比、精子活率及睾丸组织SOD活性显著降低,精子畸形率和睾丸组织MDA水平显著增高。经LSPCs预处理后辐射损伤得到改善,其中60、90 mg/kg LSPCs预处理组各项指标与ELF-EMF组相比均达到极显著差异(P<0.01)。由此提示LSPCs对ELF-EMF导致的雄性小鼠生殖损伤有明显的预防作用。
The protective effects of lotus seedpod procyanidins(LSPCs) on injury to the reproductive system in male mice, induced by extremely low-frequency electromagnetic fields(ELF-EMF). A total of 50 male, ICR mice were randomly divided into five groups: control group, ELF-EMF group, and ELF-EMF+30, 60, and 90 mg/kg LSPC groups. LSPC was administered intragastrically to the mice daily for 75 consecutive days. ELF-EMF exposure was initiated 15 days after LSPC administration began and continued for 60 days. After ELF-EMF exposure, the mice were sacrificed, and their epididymis and testes were obtained to determine the weight. Parameters such as testicle to body weight ratio, sperm motility, and malformation rates, superoxide dismutase(SOD) activity, and malondialdehyde(MDA) content were measured as well as testicular pathological changes were noted. Administration of LSPCs(especially 60 and 90 mg/kg) for 75 days effectively alleviated testicular morphological damage, significantly improved testicle to body weight ratio, sperm motility, SOD activity in the testes, reduced sperm malformation rate, and testicle MDA content. The results suggest that treatment with LSPCs could significantly alleviate reproductive injury induced by ELF-EMF in male mice.
出处
《现代食品科技》
EI
CAS
北大核心
2015年第6期13-17,共5页
Modern Food Science and Technology
基金
国家自然科学基金(31201456
31371734)
江苏省"六大人才高峰"项目(SWYY-022)
江苏省"青蓝工程"项目
江苏高校优势学科建设工程资助项目
关键词
极低频电磁场
生殖损伤
莲房原花青素
预防作用
extremely low-frequency electromagnetic field
reproductive injury
lotus seedpod procyanidins
preventive effect
