摘要
目的 对获得的抗华支睾吸虫循环抗原Fab抗体克隆进行基因分析。方法 将 2株阳性克隆抗体基因进行测序 ,并用NCBI和IgBlastAnalysisofImmunoglobulinsequences中的分析软件 ,对其推导的氨基酸序列进行分析 ,再进一步模拟它们的二级结构。结果 E38、B0 5阳性克隆抗体基因与人免疫球蛋白κ轻链和γ重链可变区基因高度同源。推导的氨基酸序列均具有典型的抗体轻、重链可变区特征。用分析软件对推测的 2株克隆轻链和重链Fd氨基酸序列进行二级结构模拟 ,结果显示以β -折叠和转角为主的平行肽链形成片层样结构 ,均符合抗体二级结构构型。 结论 E38、B0 5阳性克隆外源基因均属于人免疫球蛋白κ轻链和γ重链可变区基因。
ObjectiveTo analyze genes of Fab antibodies against Clonorchis sinensis CAg.MethodsDNA sequences of 2 positive antibody clones were determined.Analysis software of NCBI and Ig Blast Analysis of Immunoglobulin sequences were used to analyze their deduced amino acid sequences.Secondary structures of 2 positive clones' Fd fragment and light chain were also modeled.ResultsThe positive clone gene of E38 and E05 were highly homologous to the variable region of human κ light chain and γ heavy chain gene.The deduced amino acid sequences both had typical characters of Ig light and heavy chains' variable regions.The second structures was modeled with analysis software.Peptide chains containing β-sheets were found and turns formed sheet structure in the amino acid sequences of these two clones.This strucure accorded with the model of Ig secondary structure.ConclusionBoth E38 and B05 positive antibody clones belonged to κ chain and γ chains Fd variable region of human Ig gene.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第12期1418-1419,共2页
Chinese Journal of Public Health
基金
卫生部 (98 - 1 - 85)科研基金
广东省教育厅"2 1 1"工程学科建设科研基金资助
关键词
抗华支睾吸虫
循环抗原
噬菌体抗体
基因分析
二级结构
克隆
clonorchis sinensis
circulating antigen
antibody/phage
vaiable region gene
secondary structure
