摘要
为了探讨草鱼不同器官组织应对饲料途径氧化鱼油应激的差异性,以豆油、氧化鱼油为饲料脂肪源分别设计豆油组和氧化鱼油2组等氮、等能半纯化饲料,在池塘网箱养殖草鱼(平均体重(65.5±1.5)g)7 d后,采集草鱼肾脏、心脏、大脑、脾脏、鳃、肠道、皮肤、肝胰脏、肌肉共9个器官组织。采用荧光定量PCR(RT-QPCR)的方法,测定了GSH合成代谢相关酶GCLC、GSS、GSR基因表达活性,以及三个谷胱甘肽转移酶GSTω1、GSTpi、MGSt1基因的表达活性。并测定了草鱼总谷胱甘肽T-GSH在以上九种组织中的含量。结果显示,(1)经氧化鱼油刺激后,肾脏和鳃组织中T-GSH显著上升(p<0.05);(2)经氧化鱼油刺激后,GCLC在肾脏、鳃中表达量显著上调(p<0.05),GSS在肠中显著上调(p<0.05),肝胰脏显著下调(p<0.05),GSR在鳃、肠道、肝胰脏中显著上调(p<0.05),肌肉中显著下调(p<0.05);(3)经氧化鱼油刺激后,GSTω1在肠道中显著上调(p<0.05),肌肉中显著下调(p<0.05),GSTpi在肾脏中显著上调(p<0.05),心脏、肌肉中显著下调(p<0.05),MGST1在肾脏、肠道显著上调(p<0.05),脾脏显著下调(p<0.05)。结果表明,肾脏、心脏、大脑、脾脏、鳃、肠道、肝胰脏、肌肉组织都均有完整的GSH/GSTs通路。短期氧化鱼油刺激后,各个组织中GSH/GSTs通路都受到一定的影响,其中肾脏、肠道、肝胰脏、鳃的GSH/GSTs通路相对其它组织受到影响更大。
A short-term experiment was carried out to investigate the effect of oxidized fish oil on the different tissues of GSH/GSTs pathway of grass carp(Ctenopharyngodon idellus). The fish, with an initial weight of(65.5±1.5) g,was fed two diets added with soybean oil and fish oil. Collecting grass carp kidney, heart, brain, spleen, gill,intestine, skin, liver, muscles altogether nine tissues. Using RT-QPCR method to measure the expression activity of the GSH synthesis metabolizing enzymes(GCLC, GSS, GSR), and GSTs(GSTω1, GSTpi, MGSt1) gene, we also have detected the content of T-GSH in the different tissues of grass carp. Result shows that:(1) Oxidized fish oil can significantly increase(p<0.05) the content of T-GSH in kidney and gill of grass carp(p<0.05);(2) After stimulation of oxidized fish oil, GCLC was significantly increased(p<0.05) in kidney and gill of grass carp; GSS was significantly increased(p<0.05) in intestinal while the liver was significantly decreased(p<0.05). In this group grass carp GSR was significantly increased(p<0.05) in gills, intestines, liver and pancreas while the muscle was significantly decreased(p<0.05);(3) The fish stimulated by oxidized fish oil, GSTω1 was significantly increased(p<0.05) in the intestine while the muscle was significantly decreased(p<0.05). GSTpi was significantly increased(p<0.05) in the kidney while the heart and muscle were significantly decreased(p<0.05). MGST1 was significantly increased(p<0.05) in the kidney, intestine while the spleen was significantly decreased(p<0.05). In conclusion, kidneys, heart, brain, spleen, gill, intestine, liver and muscle tissue all have complete GSH/GSTs pathway. Afterthe short-term stimulus of oxidized fish oil, GSH/GSTs pathways in various tissues are all influenced, kidney, intestine, liver and gill more vulnerable than other tissue.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第11期2372-2380,共9页
Genomics and Applied Biology
基金
国家自然基金(31172417)资助
关键词
草鱼
氧化鱼油
GSH/GSTs通路
组织分布
Grass carp(Ctenopharyngodon idellus),Oxidized fish oil,GSH/GSTs,Tissue distribution
