摘要
为探究中兽药参姜止痢合剂的抗炎、镇痛作用,本试验采用二甲苯致小鼠耳廓肿胀抑制试验建立炎症模型,采用醋酸扭体试验和热板法镇痛试验建立疼痛模型,各试验造模前将昆明小鼠随机分为5个组,分别为阴性对照组(灌胃生理盐水)、阳性药物对照组[醋酸地塞米松片(抗炎),阿司匹林肠溶片(镇痛)]、参姜止痢合剂高剂量组(1 g/mL,0.4 mL)、参姜止痢合剂中剂量组(1 g/mL,0.2 mL)和参姜止痢合剂低剂量组(1 g/mL,0.1 mL),每组12只。连续预处理6 d后造模,观察并计算小鼠耳肿胀度和耳肿胀抑制率,记录小鼠扭体潜伏期,计算疼痛抑制率,测定不同检测时间点的痛阈值,以评价参姜止痢合剂的抗炎、镇痛作用。结果显示,与阴性对照组相比,中、高剂量参姜止痢合剂和阳性药物预处理6 d可极显著抑制二甲苯致小鼠耳肿胀度(P<0.01),且中剂量组耳肿胀抑制率最高,达到72%;参姜止痢合剂中、高剂量组和阳性药物对照组小鼠腹腔注射醋酸后扭体潜伏期均延长,且15 min内出现的扭体次数显著降低(P<0.05或P<0.01);中、高剂量参姜止痢合剂和阳性药物在末次预处理后1.5 h均能显著增加小鼠的痛阈值(P<0.05或P<0.01)。结果表明,参姜止痢合剂具有良好的抗炎、镇痛作用,为参姜止痢合剂的临床应用提供了参考依据。
To explore the anti-inflammatory and analgesic effects of Chinese veterinary medicine Shenjiang Zhili Mixture,this study established inflammation and pain models in mice.The inflammation model was created using xylene-induced ear swelling,and the pain model was established through the acetic acid writhing test and hot plate test.Kunming mice were randomly divided into five groups:Negative control group(gastric lavage with saline),Positive drug control group(dexamethasone acetate tablets for anti-inflammation,enteric-coated aspirin tablets for analgesia),High-dose Shenjiang Zhili mixture group(1 g/mL,0.4 mL),Medium-dose Shenjiang Zhili mixture group(1 g/mL,0.2 mL),and Low-dose Shenjiang Zhili mixture group(1 g/mL,0.1 mL),with 12 mice per group.After 6 days of continuous pre-treatment,the models were established,and ear swelling and inhibition rates were observed and calculated.The writhing latency period and pain inhibition rate were recorded,and pain thresholds were measured at different time points to evaluate the anti-inflammatory and analgesic effects of the formula.The results showed that compared with the Negative control group,the Medium and High-dose Shenjiang Zhili mixture groups and the Positive drug control group significantly inhibited xylene-induced ear swelling(P<0.01),with the Medium-dose group achieving the highest ear swelling inhibition rate(72%).In the acetic acid-induced writhing test,the latency period for writhing was prolonged,and the number of writhes within 15 minutes was significantly reduced in the Medium and High-dose Shenjiang Zhili mixture groups and the Positive drug control group(P<0.05 or P<0.01).After 1.5 hours of the final pre-treatment,the Medium and High-dose Shenjiang Zhili mixture groups and the Positive drug control group significantly increased the pain threshold(P<0.05 or P<0.01).The results indicate that the Shenjiang Zhili mixture has significant anti-inflammatory and analgesic effects,providing a reference for its clinical application.
作者
刘莲
郭志廷
张康
张景艳
王磊
李建喜
LIU Lian;GUO Zhiting;ZHANG Kang;ZHANG Jingyan;WANG Lei;LI Jianxi(Gansu Province Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine,Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs,Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China)
出处
《中国兽医杂志》
CAS
北大核心
2025年第1期128-132,共5页
Chinese Journal of Veterinary Medicine
基金
国家重点研发计划专项子课题(2022YFD1801102-2)
中国农业科学院基本科研业务费应急专项(Y2021YJ11)
中国农业科学院科技创新工程项目(25-LZIHPS-06)。
关键词
中兽药
参姜止痢合剂
抗炎
镇痛
Chinese veterinary medicine
Shenjiang Zhili mixture
anti-inflammatory
analgesia
作者简介
刘莲(1996-),男,硕士,主要从事中兽药研发工作,E-mail:ll383164095@163.com;通信作者:李建喜,E-mail:lzjianxil@163.com。
