摘要
为探究乙烯利和油菜素内酯处理下月季品种‘艾莎’对灰霉病的抗性,分别施用浓度为0、0.1、0.3和0.5 mg/L的乙烯利和油菜素内酯处理‘艾莎’花瓣,再滴加灰霉菌进行侵染,观察花瓣表型特征并使用试剂盒测定其过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢霉(CAT)活性和丙二醛(MDA)含量。结果显示:乙烯利处理下,花瓣病斑直径明显大于对照组,且随着乙烯利喷施浓度增加,POD、SOD和CAT活性先上升后下降,MDA含量显著上升;油菜素内酯处理能够抑制花瓣病斑发展,且随着油菜素内酯浸泡浓度增加,POD活性明显上升,SOD和CAT活性显著上升,MDA含量则显著下降。总体而言,乙烯利处理会降低月季品种‘艾莎’对灰霉病的抗性,而油菜素内酯能够提高其对灰霉病的抗性。
This study aims to explore the effects of ethephon and brassinolide on the resistance of Rosa chinensis'Aisha'to gray mold.The petals were treated with ethephon or brassinolide at 0(control),0.1,0.3,and 0.5 mg/L and then infected with Botrytis cinerea.The phenotypic characteristics were observed,and the activities of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and the content of malondialdehyde(MDA)were determined by kits.The results showed that the lesion diameters in the ethephon groups were larger than that in the control group.As the concentration of ethephon used for spraying increased,the activities of POD,SOD,and CAT first increased and then decreased,and the MDA content increased significantly.The brassinolide treatment inhibited the development of petal lesions.As the concentration of brassinolide used for soaking increased,the activity of POD increased obviously,and the activities of SOD and CAT increased significantly,while the content of MDA decreased significantly.In summary,ethephon attenuated the resistance of R.chinensis'Aisha'to gray mold,whereas brassinolide enhanced the resistance.
作者
邹佳梅
司满银祺
罗中元
李雪娇
毛芳
贺水莲
李耕耘
ZOU Jia-mei;SI Man-yin-qi;LUO Zhong-yuan;LI Xue-jiao;MAO Fang;HE Shui-lian;LI Geng-yun(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,PRC;Rural Industry Center of Yao'an County in Yunnan Province,Yao'an 675300,PRC)
出处
《湖南农业科学》
2024年第12期77-82,共6页
Hunan Agricultural Sciences
基金
云南省基础研究专项基金(202301AT070587)。
关键词
‘艾莎’月季
灰霉病
乙烯利
油菜素内酯
酶活性
抗病性
Rosa chinensis'Aisha'
gray mold
ethephon
brassinolide
enzyme activity
disease resistance
作者简介
邹佳梅(1999-),女,云南宣威市人,硕士研究生,主要从事观赏园艺学研究;通信作者:贺水莲;通信作者:李耕耘。
