摘要
目的:探讨真武汤通过lncRNA NEAT1/miR-31-5p/IGFBP7分子轴抑制慢性心力衰竭(CHF)进程的机制。方法:将雄性SD大鼠分为对照组、模型组、阳性对照组和真武汤低、中、高剂量组,每组各10只。除对照组外,其余各组采用腹腔注射阿霉素的方法建立CHF模型,阳性对照组给予美托洛尔灌胃,真武汤低、中、高剂量组给予真武汤(生药含量6、12、18 g/kg)灌胃。检测各组大鼠左心室射血分数(LVEF)、左心室短轴缩短率(LVFS),血清脑钠肽(BNP)水平,心脏体重比,心肌病理改变,心肌中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平及lncRNA NEAT1、miR-31-5p、IGFBP7表达水平。培养大鼠心肌H9c2细胞,采用双荧光素酶报告基因实验检测lncRNA NEAT1靶向miR-31-5p、miR-31-5p靶向IGFBP7。结果:与模型组比较,真武汤低、中、高剂量组的LVEF、LVFS增加,血清BNP水平降低(P<0.05);与模型组比较,真武汤低、中、高剂量组的心肌TNF-α、IL-1β、MDA水平降低,SOD水平增加(P<0.05);与模型组比较,真武汤低、中、高剂量组的心肌lncRNA NEAT1、IGFBP7表达降低,miR-31-5p表达增加(P<0.05)。H9c2心肌细胞中,lncRNA NEAT1靶向miR-31-5p、miR-31-5p靶向IGFBP7。结论:真武汤可能通过lncRNA NEAT1/miR-31-5p/IGFBP7分子轴改善CHF大鼠心功能并减轻炎症反应、氧化应激反应。
Objective:To investigate the mechanism of Zhenwu decoction in inhibiting the progression of chronic heart failure(CHF)through lncRNA NEAT1/miR-31-5p/IGFBP7 molecular axis.Methods:Male SD rats were divided into control group,model group,positive control group and Zhenwu decoction low-dose,medium-dose and high-dose groups with 10 rats in each group.In addition to the control group,the CHF model was established by intrabitoneal injection of adriamycin in the other groups.The positive control group was given metoprolol intragastric administration,and the Zhenwu decoction low-dose,medium-dose and high-dose groups were given Zhenwu decoction intragastric administration(crude drug content 6,12,18 g/kg).Left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS),serum brain natriuretic peptide(BNP)level,heart weight ratio,myocardial pathological changes,the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),malondialdehyde(MDA),superoxide dismutase(SOD)and the expression of lncRNA NEAT1,miR-31-5p and IGFBP7 in myocardial tissue were detected.Rat myocardial H9c2 cells were cultured,and dual luciferase reporter gene assay was used to detect lncRNA NEAT1 targeting miR-31-5p and miR-31-5p targeting IGFBP7.Results:Compared with model group,LVEF and LVFS in Zhenwu decoction low,medium and high dose groups increased,and serum BNP level decreased(P<0.05).Compared with model group,the levels of TNF-α,IL-1βand MDA decreased,while the level of SOD increased in Zhenwu decoction low,medium and high dose groups(P<0.05).Compared with model group,the expressions of lncRNA NEAT1 and IGFBP7 decreased and the expression of miR-31-5p increased in Zhenwu decoction low-dose,medium-dose and high-dose groups(P<0.05).In H9c2 cardiomyocytes,lncRNA NEAT1 targeted miR-31-5p and miR-31-5p targeted IGFBP7.Conclusion:Zhenwu decoction may improve cardiac function and reduce inflammation and oxidative stress in CHF rats through lncRNA NEAT1/miR-31-5p/IGFBP7 molecular axis.
作者
宁小康
张迎
周永学
张立
NING Xiaokang;ZHANG Ying;ZHOU Yongxue;ZHANG Li(The First School of Shaanxi University of Chinese Medcine,Xianyang 712046,China)
出处
《陕西中医》
CAS
2025年第1期8-12,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
陕西省自然科学基础研究计划项目(2024JC-YBMS-753)。
作者简介
通讯作者:张迎,E-mail:550889542@qq.com。
