摘要
目的研究当归的化学成分及其活血作用机制。方法UPLC-Q-TOF-MS鉴定化学成分;观察大鼠证侯学和心脏病理切片变化;检测血液动力学指标和蛋白质组学。结果当归共鉴定出270个化合物;给药后,大鼠弓背、耸毛,蜷缩少动,爪尾部紫暗,耳缘及鼻唇发白,饮水、进食量减少,对外界刺激反应迟钝等证候有很大改善;心肌纤维排列轻度紊乱,较模型组紧密,心肌纤维较窄,接近正常,周围可见轻度水肿、渗出,炎性细胞浸润;颈总动脉收缩压明显降低、左心室收缩压明显升高(P<0.05)。共筛选出62个差异蛋白,GO功能集中在细胞外基质、细胞骨架蛋白结合和蛋白水解的负调节等,KEGG通路富集在补体和凝血级联反应、白细胞经内皮细胞迁移及趋化因子信号通路等。结论当归可能是通过蛋白的表达,调控补体和凝血级联反应及血管平滑肌收缩等信号通路达到补血的效果。
Aim To identify the chemical components of Angelica sinensis(AS)and explore the mechanism of AS in activating blood circulation.Methods UPLC-Q-TOF-MS was used to identify the chemical components of AS.The changes of syndrome and pathological section of heart in rats were observed.Hemodynamics and proteomics were measured.Results A total of 270 compounds were identified from AS.It showed that rats of Angelica sinensis group were greatly improved such as arched back,shrugged fur,huddled up and less mobile,purplish paws and tails,whitish ear margins and nasolabial lips,reduced drinking and feeding,and slow response to external stimuli;mildly disordered myocardial fibre arrangement,myofibre arrangement was tighter than that of model group,myocardial fibres were narrower and close to normal,and mild oedema,exudation,and inflammatory cell infiltration could be seen in the surrounding area;SAP was significantly lower and LVSP was significantly higher in Angelica sinensis group(P<0.05).Proteomics showed that 62 differential proteins were screened in Angelica sinensis group compared to model,GO function were concentrated in the extracellular matrix,cytoskeletal proteins binding and protein hydrolysis negatively regulated.KEGG pathway were enriched in signalling pathways such as complement and coagulation cascades,cellular focal adhesion,leukocyte transendothelial migration and chemokine signalling pathways.Conclusions AS probably through the expression of proteins,which modulate the signalling pathways of the complement and coagulation cascade reactions and the contraction of vascular smooth muscle.
作者
焦文星
魏江霞
郭晶晶
杨志军
杨锡仓
杨秀娟
JIAO Wen-xing;WEI Jiang-xia;GUO Jing-jing;YANG Zhi-jun;YANG Xi-cang;YANG Xiu-juan(Dept of Pharmacy,Affiliated Hospital of Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;Yang Xicang national famous old Chinese medicine expert inheritance studio,Lanzhou Gansu 730000,China;Northwest China-Tibetan Medicine Co-construction and Collaborative Innovation Center,Lanzhou Gansu 730000,China;Gansu Pharmaceutical Industry Innovation Research Institute,Lanzhou Gansu 730000,China)
出处
《中国药理学通报》
CAS
北大核心
2025年第1期147-156,共10页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(82160755)
杨锡仓全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号):甘肃省教育厅产业支撑计划项目(2021CYZC-21):甘肃省教育厅项目(2021B-157)
甘肃省中医药管理局(GZKP-2022-37)
西北中藏药协同创新中心开放基金(Xbzzy-2022-08):甘肃省中药炮制技术传承基地建设揭榜挂帅项目(2022-15)。
关键词
当归
化学成分
蛋白质组学
急性血瘀证
活血作用
作用机制
Angelica sinensis
chemical components
proteomics
acute blood stasis syndrome
effect of activating blood circulation
mechanism of action
作者简介
焦文星(1985-),男,主管中药师,研究方向:中药药理学,E-mail:3500867094@qq.com;通信作者:杨秀娟(1988-),女,博士,副教授,硕士生导师,研究方向:中药及复方化学成分和药理学,E-mail:yangxiujuants@163.com。
