摘要
Pod size is a key agronomic trait that influences peanut yield greatly.However,our understanding of the mechanisms underlying pod size is limited.In this study,we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci(QTL)associated with pod size.Initial mapping performed using bulk segregant analysis revealed a candidate interval on chromosome A05 referred to as qPSW05.We refined this interval to a 256.9 kb genomic region using newly developed molecular markers.Through sequence and expression analyses,we identified the candidate gene AhXE45GC,which encodes an AN1 zinc finger protein.We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S.Accessions that lack this insertion,such as ND_L,had significantly larger pods than those with the insertion,including ND_S.To facilitate marker-assisted selection for peanut pod size,we developed a molecular marker associated with this polymorphism.This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.
基金
supported by the Key Program of National Natural Science Foundation of China (NSFC)-Henan United Fund (U22A20475)
Key Scientific and Technological Project of Henan Province (221111110500,222301420026,HARS-22-05-G1).
作者简介
Corresponding author:Dongmei Yin.E-mail address:yindm@henau.edu.cn;Kunkun Zhao,contributed equally to this work;Xufa Du,contributed equally to this work。
