摘要
为了研究GH3.17在草莓中对盐胁迫的响应,克隆了FaGH3.17,分析其进化关系、编码蛋白的理化性质等,进行烟草亚细胞定位以及拟南芥异源过表达,验证其在盐胁迫中的功能。结果表明,FaGH3.17为酸性且不稳定的疏水性非分泌性蛋白,主要定位在细胞核和细胞膜。FaGH3.17与蕨麻的亲缘关系最近。盐胁迫下,异源过表达FaGH3.17拟南芥株系的电导率、MDA含量、H2O2含量均高于野生型,分别升高了15.43%、42.97%、18.86%,而Pro含量、POD、SOD及CAT活性均显著低于野生型,分别降低了13.28%、15.42%、14.22%、19.87%,并且盐响应相关基因的相对表达量均显著降低,表明异源过表达FaGH3.17显著降低了拟南芥的抗氧化酶活性和渗透调节物质含量,预示着GH3.17可能会减弱植株的耐盐性。
In order to study the response of GH3.17 gene to salt stress in strawberry,the evolutionary relationship and physicochemical properties of FaGH3.17 were analysed using bioinformatics,and the gene was cloned and subsequently subcellularly localised in tobacco and heterologously overexpressed in Arabidopsis to verify its function in salt stress.The results showed that FaGH3.17 is an acidic and unstable hydrophobic,non-secretory protein,and the analysis of the evolutionary relationship of species revealed that FaGH3.17 is the closest relative to Potentilla anserina.Subcellular localisation in tobacco revealed that FaGH3.17 was mainly localised in the nucleus and cell membrane.Heterologously overexpression analysis in Arabidopsis thaliana revealed that under salt stress,the conductivity,MDA and H2O2 content were higher than those of wild-type plants,which were elevated by 15.43%,42.97%,and 18.86%,respectively,whereas the Pro content,POD,SOD,and CAT activities were significantly lower than those of the wild-type plants,which were reduced by 13.28%,15.42%,14.22%,19.87%,respectively.And the relative expression of salt-responsive genes were all significantly reduced.It was shown that heterologous overexpression of FaGH3.17 significantly reduced the antioxidant enzyme activities and osmoregulatory substance contents in Arabidopsis,which predicts that GH3.17 may attenuate the salt tolerance of plants.
作者
杨娟博
郭丽丽
卢世雄
苟惠敏
王帅珽
曾宝珍
毛娟
YANG Juanbo;GUO Lili;LU Shixiong;GOU Huimin;WANG Shuaiting;ZENG Baozhen;MAO Juan(Collage of Horticulture,Gansu Agricultural University,Lanzhou 730000,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2024年第11期2483-2494,共12页
Acta Horticulturae Sinica
基金
甘肃省自然基金重点基金项目(22JR5RA831)。
关键词
草莓
基因克隆
盐胁迫
功能验证
strawberry
gene cloning
salt stress
functional verification
作者简介
通信作者:毛娟,(E-mail:maojuan@gsau.edu.cn。
