摘要
为明确UCP基因SNP与坝上长尾鸡脂肪酸性状间的关联性,为坝上长尾鸡脂肪酸性状分子标记辅助育种奠定基础,本试验采用直接测序技术对已测肌肉脂肪酸的54只坝上长尾鸡进行UCP基因SNP检测,运用SPSS 26.0软件中一般线性模型对SNP与坝上长尾鸡脂肪酸性状进行关联性分析。通过直接测序,在坝上长尾鸡UCP基因片段上共检测出10个SNP位点,包含3个中度多态位点和7个低度多态位点,且10个位点均符合哈代-温伯格平衡(P>0.05)。3个位点含有3种基因型,7个位点含有2种基因型。关联分析结果显示,g.224A>T、g.284C>T、g.329G>A、g.409T>C和g.450G>A各基因型与脂肪酸含量无显著相关(P>0.05);g.195A>G各基因型与棕榈油酸、γ亚麻酸和肉豆蔻油酸呈显著相关(P<0.05);g.398C>T各基因型与肉豆蔻油酸、珠光脂酸和二高γ亚麻酸呈显著相关(P<0.05);g.399A>G各基因型与珠光脂酸呈显著相关(P<0.05);g.431C>T各基因型与肉豆蔻油酸、珠光脂酸和二高γ亚麻酸呈显著相关(P<0.05);g.434C>T各基因型与反油酸呈显著相关(P<0.05)。综上所述,g.195A>G、g.398C>T、g.399A>G、g.431C>T和g.434C>T,5个位点与不同类型脂肪酸含量呈显著相关(P<0.05),可作为坝上长尾鸡分子标记辅助育种的候选标记。
The correlation between UCP gene SNP and the fatty acid traits of Bashang long-tailed chickens was clarified,which laid a foundation for molecular marker-assisted breeding of fatty acid traits in Bashang long-tailed chickens.Direct sequencing was used to detect UCP gene SNP in 54 longtail chickens with measured muscle fatty acids.The general linear model in SPSS 26.0 software was used to analyze the correlation between SNP and the fatty acid traits of longtail Bashang chickens.Through direct sequencing,a total of 10 SNP sites were detected on the UCP gene fragment of Bashang long-tailed chicken and 10 sites all conformed to the Hardy-Weinberg equilibrium(P>0.05).3 loci contained 3 genotypes,and 7 loci contained 2 genotypes.The correlation analysis results showed that the genotypes of g.224A>T,g.284C>T,g.329G>A,g.409T>C and g.450G>A were not significantly correlated with fatty acid content(P>0.05).The genotypes of g.195A>G were significantly correlated with palmitoleic acid,γ-linolenic acid and myristic oleic acid(P<0.05),g.398C>T genotypes were significantly correlated with myristic oleic acid,pearlescent acid and DH-γ-linolenic acid(P<0.05),g.399A>G genotypes were significantly correlated with myristic acid(P<0.05),and g.431C>T genotypes were significantly correlated with myristic oleic acid,pearlescent acid and DH-γ-linolenic acid(P<0.05);g.434C>T genotypes were significantly associated with antioleic acid(P<0.05).Therefore it is concluded that the five loci are significantly correlated with the content of different types of fatty acids(P<0.05),which can serve as the candidate markers for molecular marker-assisted breeding of Bashang long-tail chicken.
作者
胡紫意
李可强
李祥龙
HU Ziyi;LI Keqiang;LI Xianglong(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004;College of Mathematics and Information Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004;Key Laboratory of Exploration and Innovation of Characteristic Animal Germplasm Resources of Hebei Province,Qinhuangdao,Hebei 066004,China)
出处
《家畜生态学报》
北大核心
2024年第12期16-22,共7页
Journal of Domestic Animal Ecology
基金
河北省鸡现代种业科技创新团队坝上长尾鸡挖掘与精准鉴定(21326303D-6)和鸡智能育种技术研究(21326303D-7)
作者简介
胡紫意(1999-),女,河北刑台人,硕士研究生,研究方向:动物遗传育种与繁殖。E-mail:1405374138@qq.com;通讯作者:李祥龙(1963-),男,河北丰南人,博士,教授,主要从事动物遗传育种研究。E-mail:lixianglongcn@yahoo.com。
