摘要
研究旨在优化振荡提取乌梅中柠檬酸工艺并对其黄花菜着色研究。采用高效液相色谱法测定柠檬酸含量,以柠檬酸的总含量为考察指标,基于单因素(时间、温度、振荡频率和料液比)试验结果,利用Box-Behnken设计-响应面法优化提取工艺,将乌梅提取液应用于黄花菜着色。结果表明,乌梅最优的提取工艺为温度60℃、提取时间60 min,振荡速度为261 r/min以及料液比为0.067,此工艺条件下,乌梅柠檬酸得率可达到17.5%,与模型预测值相比相对误差为1.44%,优化后工艺条件实用性强、可信度高。黄花菜的着色试验表明:当乌梅提取液中柠檬酸的浓度为3.4%-8.5%时对水黄花菜的着色效果较好。总之,振荡提取法提取乌梅中柠檬酸的工艺简单、得率高,提取液呈现较好的着色效果,为乌梅柠檬酸的深入研究和应用开发提供了一定的参考。
This study aims to optimize the process of oscillating extraction of citric acid from dark-plum and study its coloring effect on day lily.The content of citric acid was determined by high-performance liquid chromatography.Using the total content of citric acid as the index,based on the results of single-factor experiments(time,temperature,oscillation frequency,and material-to-liquid ratio),the Box-Behnken design-response surface method was used to optimize the extraction process,and the extract of dark-plum was applied to the coloring of day lily.The results showed that the optimal extraction process of dark-plum was at a temperature of 60℃,an extraction time of 60 min,an oscillation speed of 261 r/min,and a material-to-liquid ratio of 0.067.Under these conditions,the yield of citric acid from dark-plum could reach 17.5%,with a relative error of 1.44%compared with the model prediction.The optimized process conditions were practical and reliable.The coloring experiment of day lily showed that the coloring effect of dark-plum extract with a citric acid concentration of 3.4%-8.5%was better on water day lily.In conclusion,the oscillating extraction method for extracting citric acid from dark-plum is simple and has a high yield.The extract exhibits good coloring effects,providing a certain reference for further research and application development of citric acid from dark-plum.
作者
蔡光容
蔡纹素
龚燕川
董雪
CAI Guangrong;CAI Wensu;GONG Yanchuan;DONG Xue(Key Laboratory of Characteristic Plant Development and Research in Sichuan Provincial Universities,Dazhou Sichuan 635000,China;College of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou Sichuan 635000,China)
出处
《佳木斯大学学报（自然科学版）》
CAS
2024年第10期161-164,175,共5页
Journal of Jiamusi University：Natural Science Edition
基金
四川省中医药管理局科技项目(2020JC0117)
特色植物开发研究四川省高校重点实验室(TSZW2001)
四川文理学院校级科技项目(2019PT004Z)
四川文理学院博士启动资金(2021RC002Z)。
关键词
乌梅
柠檬酸
响应面分析
黄花菜
着色
dark-plum
citric Acid
response surface analysis
day lily
coloring
作者简介
蔡光容(1983-),女,贵州遵义人,副研究员,博士,研究方向:天然产物化学及植物逆境生理生化。
