摘要
铜对植物生长至关重要,过量会导致生物量降低。AtVIP1属于拟南芥bZIP家族,具有淀粉积累、渗透信号传导和促进农杆菌转化等作用。转录因子VIP1在植物响应非生物胁迫方面已有研究报道,但与铜胁迫相关的研究还未见报道。为明确AtVIP1基因过表达植株在响应铜胁迫过程中的作用,向1/2 MS固体培养基中加入50μM的铜离子,观察AtVIP1基因过表达植株在胁迫后对种子萌发及幼苗生长的影响。结果表明:在铜胁迫下,AtVIP1基因过表达可减轻对种子萌发的抑制,缓解幼苗受到的伤害,提高SOD、POD和CAT等抗性酶的活性,减少MDA含量,降低细胞膜的通透性,增强拟南芥对铜胁迫的抗性。综上,研究结果为深入解析植物响应铜胁迫的分子调控网络提供一定的理论依据。
Copper is crucial for plant growth,and excessive amounts can lead to a decrease in biomass.AtVIP1 belongs to the bZIP family of Arabidopsis thaliana,which has the functions of starch accumulation,osmotic signaling and promoting Agrobacterium transformation.The transcriptional factor VIP1 has been reported in plant response to abiotic stress,but the study related to copper stress has not been reported.To clarify the role of overexpression of AtVIP1 gene in the response of plants to copper stress,50μM copper ion was added to 1/2 MS solid medium to observe the effects of overexpression of AtVIP1 gene on seed germination and seedling growth after stress.The results showed that under copper stress,overexpression of AtVIP1 gene could alleviate the inhibition of seed germination,alleviate the damage to seedling growth,increase the activity of resistant enzymes such as SOD,POD and CAT,reduce MDA content,reduce cell membrane permeability,and enhance the resistance of Arabidopsis thaliana to copper stress.The results could provide a theoretical basis for further analysis of the molecular regulatory network of plant response to copper stress.
作者
李学影
李征杭
赵阳
王鑫
李思琪
黄荣臻
徐海军
戴凌燕
Li Xueying;Li Zhenghang;Zhao Yang;Wang Xin;Li Siqi;Huang Rongzhen;Xu Haijun;Dai Lingyan(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;Daqing Branch of Heilongjiang Academy of Sciences)
出处
《黑龙江八一农垦大学学报》
2024年第6期79-85,92,共8页
journal of heilongjiang bayi agricultural university
基金
黑龙江省自然科学基金联合引导项目(LH2022C062)
黑龙江省省属科研院所科研业务费项目(CZKYF2022-1-B025)。
关键词
铜胁迫
拟南芥
AtVIP1
过表达
生长发育
抗性
copper stress
arabidopsis thaliana
AtVIP1
overexpression
growth and development
resistance
作者简介
李学影(1997-),女,黑龙江八一农垦大学生命科学技术学院2021级硕士研究生;通信作者:戴凌燕,女,教授,博士研究生导师,E-mail:dailingyan770416@126.com。
