摘要
水稻(Oryza sativa L.)是重要的粮食作物,其植株光合作用与肥料利用的效率是决定水稻产量的重要因素。本研究在水稻‘科辐粳7号’的辐射诱变群体中筛选到一个叶片黄化且分蘖减少的突变体,并将其命名为yllt10(yellow leaf and less tillering 10)。与野生型对照相比,该突变体叶片的叶绿素含量降低、叶绿体的结构发育异常、光合作用速率显著下降。遗传分析表明yllt10突变体的表型受单隐性核基因控制,利用图位克隆技术将YLLT10基因定位于水稻第10号染色体J4与J5两个分子标记之间,通过对该区间内的注释基因进行PCR测序,发现在yllt10突变体中CAO1/PGL基因的第1个外显子发生单碱基缺失,从而导致该基因的移码突变,是CAO1/PGL基因的一个新等位变异。此外,在不同氮素浓度种植条件下,yllt10突变体均表现出对氮素不敏感的表型。本研究表明YLLT10基因调控水稻的叶色和分蘗数,影响水稻的光合作用和产量,对该基因的功能机制研究能够为水稻高产育种提供一定的理论依据。
Rice(Oryza sativa L.)is a major food crop and increasing rice yield is the primary objective of rice research.Photosynthesis and nitrogen utilization efficiency directly affect the tiller number of rice,which affects the yield of rice.In this study,a stable yellow leaf and less tillering rice mutant yllt10(yellow leaf and less tillering 10)was obtained by heavy-ion beam mutagenesis of rice variety‘Ke-fu-geng 7’.Compared with the wild type,yllt10 showed reduced chlorophyll content,decreased photosynthesis rate,and abnormal chloroplast structure.The genetic analysis indicated that the phenotype of yllt10 was controlled by a single recessive nuclear gene.Map-based cloning localized YLLT10 between two molecular markers J4 and J5 on chromosome 10.The sequencing of candidate genes within this interval revealed that YLLT10 was an allelic mutation of CAO1/PGL with a single base deletion in the first exon resulting in the frame shift mutation of CAO1/PGL,and YLLT10 was a new allelic variation of CAO1/PGL.The mutant yllt10 was insensitive to changes in nitrogen concentration when being incubated with different nitrogen concentrations.YLLT10 controls leaf color and tiller number and affects photosynthesis and yield of rice.The study of this gene provides a theoretical basis for molecular breeding of rice.
作者
李文浩
朱晓彤
罗会超
彭令灵
詹玥
叶亚峰
吴跃进
陶亮之
马伯军
陈析丰
刘斌美
LI Wenhao;ZHU Xiaotong;LUO Huichao;PENG Lingling;ZHAN Yue;YE Yafeng;WU Yuejin;TAO Liangzhi;MA Bojun;CHEN Xifeng;LIU Binmei(College of Life Sciences,Zhejiang Normal University,Jinhua 321004,Zhejiang,China;Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,Anhui,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2024年第10期3471-3484,共14页
Chinese Journal of Biotechnology
基金
安徽省自然科学基金(2108085MC99)
合肥市“借转补”科技专项(J2020G45)
安徽省科技重大专项(202003c08020006)
浙江省自然科学基金重点项目(LZ23C130004)。
关键词
水稻突变体
叶片黄化
少分蘖
氮素响应
rice mutant
leaf yellowing
less tillering
nitrogen response
作者简介
Corresponding authors:CHEN Xifeng,xfchen@zjnu.cn;Corresponding authors:LIU Binmei,liubm@ipp.ac.cn。
