摘要
从铜污染地区筛选到一株高铜抗性菌株,经过18S和26S rRNA测序分析,与罗伦隐球酵母亲缘关系最近.经测试发现菌株能在15mmol/L Cu^(2+)的培养基中正常生长,最高吸附铜29.36μmol/L/OD.另外,对菌株进行铜应激后做转录组分析发现共有126个基因表达上调,143个基因表达下调;多个氨基酸代谢、能量代谢、金属离子转运途径有关基因出现差异表达.同时,铜应激导致糖代谢途径受阻,相关基因表达量下降37%~73%,这可能是铜抑制菌生长的重要原因之一;重金属转运相关功能基因在铜诱导时,表达量急剧上升7~26倍,以应对铜胁迫.本研究揭示菌株对铜胁迫的转录反应和可能参与铜抗性的代谢途径,为通过微生物技术应用于铜污染修复提供依据.
We isolated a copper-resistant strain from a copper-contaminated area.The 18S and 26S rRNA sequencing showed it had the closest genetic relationship with Cryptococcus laurentii.It was found that the strain could grow normally in the medium with 15mmol/L Cu^(2+),and the maximum adsorption of copper was 29.36μmol/L/OD.In addition,the transcriptome analysis of the strains after copper stress showed that 126genes were up-regulated and 143genes were down-regulated;multiple genes related to amino acid metabolism,energy metabolism,and metal ion transport pathways were differentially expressed.At the same time,we found that copper stress blocked the glucose metabolism pathway,the expression of metabolism related genes decreased by 37%to 73%,which may be one of the important reasons why copper inhibited the growth of bacteria.The expression of heavy metal transport-related functional genes increased dramatically by 7 to 26 times in response to copper stress when induced by copper.The purpose of this study is to reveal the transcriptional response of the strain to copper stress and the metabolic pathways that may be involved in copper resistance,and to provide a basis for the application of microbial technology in copper pollution remediation.
作者
刘文信
温群伟
周旭
吴清文
徐阳
秦春燕
陈雪岚
黄明珠
LIU Wen-xin;WEN Qun-wei;ZHOU Xu;WU Qing-wen;XU Yang;QIN Chun-yan;CHEN Xue-lan;HUANG Ming-zhu(Natl R&D Ctr Freshwater Fish Proc,Jiangxi Normal University,Nanchang 330022,China;School of Life Science,Jiangxi Normal University,Nanchang 330022,China;School of Health,Jiangxi Normal University,Nanchang 330022,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第11期6271-6277,共7页
China Environmental Science
基金
江西省自然科学基金资助面上项目(20232BAB205003)
江西省自然科学基金资助青年项目(20224BAB215004)
江西省自然科学基金资助重点项目(20202ACBL205001)
国家自然科学基金资助项目(32260014)。
关键词
铜耐受
铜吸附
转录组
能量代谢
金属离子转运
copper tolerance
copper adsorption
transcriptome
energy metabolism
metal ion transport
作者简介
黄明珠,助理研究员,huangmingzhu12@163.com;刘文信(2001-),男,江西九江人,江西师范大学硕士研究生,主要从事微生物吸附重金属研究.发表论文1篇.798158794@qq.com.
