摘要
目的探究食管癌(EC)组织Twist1表达对EC患者临床病理特征及预后的影响。方法选取EC患者150例作为观察组,同时选取同期正常食管非肿瘤性病变组织标本150例作为对照组。通过免疫组化法检测并比较2种组织标本中Twist1的阳性表达率,应用免疫印迹法检测2种组织中Twist基因的表达水平,并分析EC组织中Twist1的阳性表达与临床病理特征的关系。结果与正常食管非肿瘤性病变组织相比,癌组织中的Twist1阳性表达率及Twist1相对表达量均显著升高(P<0.05)。Twist1的阳性表达与EC患者的性别、年龄、肿瘤直径、病理类型以及分化程度均无关(P>0.05);在TNM分期为Ⅲ~Ⅳ期、有淋巴结转移以及有远处转移的患者中阳性表达率更高(P<0.05)。结论Twist1在EC组织中呈高表达状态,与TNM分期Ⅲ~Ⅳ期、淋巴结转移及远处转移具有一定相关性。检测Twist1的表达水平,有助于EC的早期诊断,同时还可为临床上判断EC患者的预后提供参考。
Objective To investigate the effect of Twist1 expression in esophageal cancer(EC)tissues on clinicopathological features and prognosis of EC patients.Methods 150 cases of EC patients were selected as the observation group,and 150 cases of normal esophageal non-neoplastic lesion tissue specimens were selected as the control group in the same period.The positive expression rate of Twist1 in the 2 tissue specimens was detected and compared by immunohistochemistry,the expression level of Twist gene in the 2 tissues was detected by applying immunoblotting,and the relationship between the positive expression of Twist1 in EC tissues and clinicopathological features was analyzed.Results Compared with normal esophageal non-neoplastic lesion tissues,the positive expression rate of Twist1 and the relative expression of Twist1 were significantly higher in cancer tissues(P<0.05).The positive expression of Twist1 was not related to the gender,age,tumor diameter,pathological type and differentiation degree of EC patients(P>0.05);in patients with TNM stageⅢ~Ⅳ,lymph node metastasis and patients with distant metastasis(P<0.05).Conclusion Twist1 is highly expressed in EC tissues,which was correlated with TNM stageⅢ~Ⅳ,lymph node metastasis and distant metastasis.Detecting the expression level of Twist1 in the clinical treatment of EC patients can help in the early diagnosis of EC cancer,and also provide a reference for judging the prognosis of EC patients in the clinic.
作者
袁艺
岳颖
姚晨
YUAN Yi;YUE Ying;YAO Chen(The Second People's Hospital of Henan Province,Zhengzhou,451191)
出处
《实用癌症杂志》
2024年第11期1759-1761,1767,共4页
The Practical Journal of Cancer
