摘要
以紫外光谱、荧光光谱和分子对接等方法探究玉米醇溶蛋白和槲皮素分子之间在醇溶体系内的相互作用机制,并通过粒径、包埋率及微观形貌等指标对其形成的纳米颗粒进行表征,初步探讨不同质量比的槲皮素与玉米醇溶蛋白对纳米颗粒的影响。结果表明,槲皮素与玉米醇溶蛋白发生了荧光静态猝灭,作用位点更接近玉米醇溶蛋白的色氨酸残基,其分子间相互作用力为氢键和范德华力。分子对接结果显示,槲皮素与玉米醇溶蛋白的氨基酸残基ALA-127、LEU-81、PRO-161、LEU-156形成疏水作用力,与SER-159和GLN-134残基形成氢键。玉米醇溶蛋白与槲皮素质量比为20∶1时,形成了具有球状形貌核壳结构的纳米颗粒,且其粒径为88.31 nm,电位为35.96 mV,包埋率为81.09%。
The UV spectrum,fluorescence spectrum,and molecular docking were used to investigate the interaction mechanism between quercetin and zein in ethanol-water solution.Additionally,the micro-morphology,entrapment ratio,and particle size,etc.of the composite colloidal particles were determined to preliminary investigation how varied mass ratios of quercetin and maize gliadin affect nanoparticles.The findings revealed that both quercetin and zein demonstrated fluorescence static quenching,with zein s tryptophan residue serving as the action site.Van der Waals forces and hydrogen bonding were implicated in the intermolecular interaction.Molecular docking analysis indicated that quercetin formed hydrophobic interactions with amino acid residues ALA-127,LEU-81,PRO-161,and LEU-156 of zein,as well as hydrogen bonds with residues SER-159 and GLN-134.When the mass ratio of zein to quercetin was 20∶1,core-shell nanoparticles were formed,characterized by a particle size of 88.31 nm,a potential of 35.96 mV,and an embedding rate of 81.09%.
作者
王金霞
王家薇
王开俊
吴长玲
吴峰华
刘兴泉
王鹏
Wang Jinxia;Wang Jiawei;Wang Kaijun;Wu Changling;Wu Fenghua;Liu Xingquan;Wang Peng(College of Food and Health,Zhejiang Agriculture and Forestry University,Hangzhou 311300)
出处
《中国粮油学报》
CAS
CSCD
北大核心
2024年第9期163-171,共9页
Journal of the Chinese Cereals and Oils Association
基金
疏水性功能因子纳米载运体系构筑及其互作机制研究(2022LFR122)。
作者简介
第一作者:王金霞,女,2000年出生,硕士,食品加工与安全,1668485444@qq.com;通信作者:王鹏,女,1992年出生,副教授,森林食物资源开发与利用,wpeng@zafu.edu.cn。
