摘要
目的探讨基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)快速检测肺炎克雷伯菌和铜绿假单胞菌产碳青霉烯酶的临床价值。方法收集2022年1月至2023年10月徐州医科大学附属邳州市人民医院分离的肺炎克雷伯菌60株和铜绿假单胞菌80株,其中碳青霉烯类耐药肺炎克雷伯菌(CRKP)30株,碳青霉烯类敏感肺炎克雷伯菌(CSKP)30株,碳青霉烯类耐药铜绿假单胞菌(CRPA)50株,碳青霉烯类敏感铜绿假单胞菌(CSPA)30株。分别采用改良碳青霉烯灭活试验(mCIM)、胶体金免疫层析法和Autof MS 1000质谱鉴定系统进行检测,评估Autof MS 1000质谱鉴定系统检测肺炎克雷伯菌和铜绿假单胞菌产碳青霉烯酶的能力。结果Autof MS 1000质谱鉴定系统检测结果与mCIM和胶体金免疫层析法结果完全一致,30株CRKP中,有28株检测到碳青霉烯酶,2株阴性;50株CRPA中,有15株检测到碳青霉烯酶,35株阴性;30株CSKP和30株CSPA均为阴性。3种方法检测碳青霉烯酶的结果符合率为100%。结论Autof MS 1000质谱鉴定系统检测碳青霉烯酶的结果与mCIM法和胶体金免疫层析法一致,且既有mCIM法低成本的特点,又有胶体金免疫层析技术速度快、准确率高的优点,可用于临床肺炎克雷伯菌和铜绿假单胞菌产碳青霉烯酶菌株的快速鉴定。
Objective To investigate the clinical value of matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS)in rapid detection of carbapenemase produced by Klebsiella pneumoniae and Pseudomonas aeruginosa.Methods A total of 60 strains of Klebsiella pneumoniae and 80 strains of Pseudomonas aeruginosa isolated from Pizhou People's Hospital affiliated to Xuzhou Medical University from January 2022 to October 2023 were collected,including 30 strains of carbapenem-resistant Klebsiella pneumoniae(CRKP),30 strains of carbapenem-sensitive Klebsilla pneumoniae(CSKP),50 strains of carbapenem-resistant Pseudomonas aeruginosa(CRPA)and 30 strains of carbapenem-sensitive Pseudomonas aeruginosa(CSPA).Three detection methods were applied,i.e.,modified carbapenem inactivation method(mCIM),colloidal gold immunochromatography and Autof MS 1000 mass spectrometry identification system to evaluate the ability of Autof MS 1000 mass spectrometry identification system in detecting carbapenase production of Klebsiella pneumoniae and Pseudomonas aeruginosa.Results The results of Autof MS 1000 mass spectrometry identification system were consistent with those of both mCIM and colloidal gold immunochromatography.Carbapenemase was detected in 28 of the 30 CRKP strains,and it was negative in 2 CRKP strains.Carbapenamase was detected in 15 of the 50 CRPA strains and it was negative in 35 CRPA strains.Thirty strains of CSKP and 30 strains of CSPA were all Carbapenemase negative.The coincidence rate of the results of the three methods in the detection for carbapenase was 100%.ConclusionThe result of Autof MS 1000 mass spectrometry identification system has been consistent with those of mCIM and colloidal gold immunochromatography.It not only has the characteristics of cost-saving compare with of mCIM method,but also hold the advantages of fast speed and high accuracy of colloidal gold immunochromatography method.Thus,Autof MS 1000 system can be used for the rapid identification of carbapenemase produced by Klebsiella pneumoniae and Pseudomonas aeruginosa.
作者
陆丹
沈艳丽
魏望
周雪婷
曹宇杰
潘倩
薛魁
LU Dan;SHEN Yanli;WEI Wang;ZHOU Xueting;CAO Yujie;PAN Qian;XUE Kui(Department of Clinical Laboratory,Pizhou People's Hospital,Xuzhou Medical University,Pizhou 221300,Jiangsu,China)
出处
《临床检验杂志》
CAS
2024年第10期744-747,共4页
Chinese Journal of Clinical Laboratory Science
基金
江苏省科技计划项目(Z2022056)
徐州市卫健委青年医学科技创新项目(XWKYHT20220160)。
关键词
基质辅助激光解吸电离飞行时间质谱
碳青霉烯酶
肺炎克雷伯菌
铜绿假单胞菌
matrix-assisted laser desorption/ionization time of flight mass spectrometry
carbapenemase
Klebsiella pneumoniae
Pseudomonas aeruginosa
作者简介
陆丹,1992年生,女,主管技师,硕士研究生,主要从事临床细菌耐药机制研究;通信作者:薛魁,主任技师,E-mail:15716706336@163.com。
