摘要
目的探索原花青素对重度意外性低体温致小鼠急性肺损伤的保护作用及机制。方法将45只小鼠分为空白组(CON组,n=15)、模型组(CE组,n=15)和干预组(CE+PCs组,n=15)。CON组和CE组10 ml/kg蒸馏水灌胃7 d,CE+PCs组采用500 mg/kg原花青素灌胃7 d,CE组及CE+PCs组在第8天建立重度意外性低体温致小鼠肺损伤模型后处死。观察各组小鼠肺组织大体改变,肺组织病理学改变并进行肺损伤评分;计算肺组织湿干比;ELISA法检测肺组织匀浆中TNF-α及IL-6的浓度;蛋白免疫印迹法检测肺组织TLR4、NFκB-P65及IκBα表达。结果CE组小鼠肺损伤评分高于CON组;CE+PCs组小鼠肺损伤评分低于CE组;CE组小鼠肺湿干比高于CON组;CE+PCs组小鼠肺湿干比低于CE组;CE+PCs组小鼠肺损伤评分低于CE组;CE组小鼠肺组织匀浆中的TNF-α和IL-6浓度高于CON组;CE+PCs组小鼠肺组织匀浆中TNF-α和IL-6浓度低于CE组;CE组小鼠肺组织中TLR4及NFκB-P65表达均高于CON组,IκBα表达低于CON组;CE+PCs组小鼠肺组织中TLR4及NFκB-P65表达低于CE组,IκBα表达水平高于CE组,差异均有统计学意义(P<0.05)。结论重度意外性低体温可导致小鼠急性肺损伤,该过程可能与炎症通路的激活有关。原花青素可能通过抑制TLR4/NFκB通路减少炎症反应来保护重度意外性低体温诱导的急性肺损伤。
Objective Explore the protective effect and mechanism of proanthocyanidins on acute lung injury in mice caused by severe accidental hypothermia.Methods 45 mice were randomly divided into three groups of 15 mice each,namely,the CONoperated group(CON group),the model group(CE group),and the intervention group(CE+PCs group)group.The CoN group and CE group was administered by gavage 10 mg/kg distilled water for 7 consecutive da The CE+PCs group was administered by gavage 500 mg/kg proanthocyanidin solution once daily for 7 consecutive days.CE group and CE+PCs group were killed on the 8th day after establishing lung injury model caused by severe accidental hypothermia.The general changes of lung tissue in each group were observed.Lung histopathological changes were observed and lung injury score was performed.The wet dry ratio of pulmonary was calculated by weighing them.ELISA was used to detect the concentrations of TNF-αand IL-6 in lung tissue homogenates.Additionally,Western blot was used to measure the expression levels of TLR4,NFκB-P65 and IκBαin lung tissues.Results The lung injury score of CE group was higher than that of CON group.The lung injury score in CE+PCs group was lower than that in CE group.The lung wet dry ratio in CE group was lower than that in CON group.The lung wet dry ratio in CE+PCs group was lower than that in CE group.The lung injury score of CE+PCs group was lower than that of CE group.The concentrations of TNF-αand IL-6 in lung homogenate of mice in CE group were higher than those in CON group.The concentrations of TNF-αand IL-6 in lung homogenate of mice in CE+PCs group were lower than those in CE group.The expression of TLR4 and NFκB-P65 in lung tissue of CE group was higher than that of CON group,and the expression of IκBαwas lower than that of CON group.The expression of TLR4 and NFκB-P65 in lung tissues of CE+PCs group was lower than that of CE group,and the expression level of IκBαwas higher than that of CE group,the differences were statistically significant(P<0.05).Conclusion Severe accidental hypothermia can lead to acute lung injury in mice,a process that may be associated with activation of inflammatory pathways.Proanthocyanidins protect against severe accidental hypothermia-induced acute lung injury by inhibiting the TLR4/NFκB pathway and reducing the inflammatory response.
作者
曹滨验
戴晶
游平飞
胡安
于新辉
金红旭
刘颖
CAO Bin-yan;DAI Jing;YOU Ping-fei;HU An;YU Xin-hui;JIN Hong-xu;LIU Ying(Department of Emergency Medicine,General Hostipal of Northern Theater Command,Shenyang 110016,China)
出处
《创伤与急危重病医学》
2024年第5期267-271,共5页
Trauma and Critical Care Medicine
作者简介
第一作者:曹滨验(1998-),女,辽宁辽阳人,硕士;通信作者:刘颖,E-mail:cszx_ly@163.com。
