摘要
本试验以番茄栽培品种‘Heinz 1706’为试材,研究了甜菜糖蜜处理对番茄幼苗生长和糖转运的影响,以期为甜菜糖蜜的推广应用提供理论依据。试验共设2个处理:以番茄喷施水为对照组,以番茄喷施等量甜菜糖蜜(稀释300倍)为处理组。与对照组相比,甜菜糖蜜处理使番茄幼苗的株高、茎粗、冠幅、叶片面积、根表面积、根体积、根长、根平均直径、地上部干重、地上部鲜重、地下部干重、地下部鲜重、叶绿素含量和净光合速率分别增加了27.2%、10.6%、20.6%、61.8%、20.3%、25.3%、24.2%、11.0%、25.1%、26.3%、21.4%、33.2%、17.2%和32.7%。此外,本研究测定了糖和蔗糖转运蛋白(sucrose transporter, SUT)基因在源库组织(茎尖、茎、叶片和根部)中的表达模式。与对照组相比,甜菜糖蜜处理使番茄中糖含量显著增加,尤其是在叶片和茎尖中分别积累了大量的蔗糖和还原性糖。SlSUTs基因在番茄幼苗的茎尖、茎、叶片和根部的表达显著上调。综上可知,甜菜糖蜜可能是通过诱导SlSUTs表达,促进番茄幼苗源库组织中糖的积累和高效分配,增加叶绿素含量及光合能力,最终促进了番茄的生长。
The experiment utilized the tomato cultivar‘Heinz 1706’to investigate the impact of beet molasses treatment on the growth and sugar transport of tomato seedlings,aiming to provide a theoretical basis for the widespread application of beet molasses.Two treatments in the experiment:the control group received water sprayed on tomato plants,and the treatment group received an equivalent amount of beet molasses(diluted 300 times)sprayed on tomato plants.Compared with the control group,sugar beet molasses treatment increased the plant height,stem diameter,crown width,leaf area,root surface area,root volume,root length,average root diameter,aboveground dry weight,aboveground fresh weight,underground dry weight,underground fresh weight,Chlorophyll content and net photosynthetic rate of tomato seedlings by 27.2%,10.6%,20.6%,61.8%,20.3%,25.3%,24.2%,11.0%,25.1%,26.3%,21.4%,33.2%,17.2%and 32.7%,respectively.Further,this study assessed the expression patterns of sugar content and sucrose transporter(SUTs)genes in source and sink(stem tip,stem,leaves,and roots).The results indicated that beet molasses treatment significantly increased sugar content in tomato seedlings,particularly accumulating substantial sucrose and reducing sugars in leaves and stem tip.Compared with the control group,sugar content in tomato increased significantly,especially in the leaves and shoot tips accumulated a lot of sucrose and reducing sugar,respectively.The expression of SLSUTS gene was significantly up-regulated in stem tip,stem,leaf and root of tomato seedlings.In summary,beet molasses may induce the expression of tomato SlSUTs genes,promoting the accumulation and efficient distribution of sugars in source and sink of tomato seedlings.This enhancement increased chlorophyll content and photosynthetic capacity,ultimately stimulating tomato growth.
作者
朱建伟
李佳琦
刘硕
陈春燕
刘根忠
张旭
马方放
包志龙
ZHU Jian-wei;LI Jia-qi;LIU Shuo;CHEN Chun-yan;LIU Gen-zhong;ZHANG Xu;MA Fang-fang;BAO Zhi-long(College of Horticulture Science and Engineering/Shandong Agricultural University,Tai'an 271018,China)
出处
《山东农业大学学报（自然科学版）》
北大核心
2024年第5期757-766,共10页
Journal of Shandong Agricultural University：Natural Science Edition
基金
国家自然科学基金(31872951)。
作者简介
第一作者:朱建伟(1997-),男,硕士研究生,研究方向:蔬菜分子生物学。E-mail:564585038@qq.com;通讯作者:马方放,E-mail:fma@sdau.edu.cn;通讯作者:包志龙,E-mail:zbao@sdau.edu.cn。
