摘要
中肋骨条藻(Skeletonema costatum)是一种全球海域广布种与赤潮多发种,对其进行检测与监测对海洋生态环境研究具有重要意义。本研究以核酸快速提取方法及环介导恒温扩增技术为基础,建立了一种针对中肋骨条藻的快速可视化现场检测方法。该方法特异性强、灵敏度高(可达1.2 pg基因组DNA)、检测时间短(100 min以内),可通过反应体系颜色变化对藻细胞有无进行快速判断。该方法经过大量野外样品的验证,具有较高的可行性,可被推广应用于中肋骨条藻的现场监测工作中。
Skeletonema costatum is a widely distributed species in global waters,which also causes red tide frequently.It is of great significance to detect and monitor S.costatum for marine ecological environment research.In this study,a rapid visual in-situ detection method for S.costatum was developed based on rapid nucleic acid extraction and loop-mediated isothermal amplification.This method offers the advantages of high specificity,high sensitivity(1.2 pg genomic DNA)and rapid detection time(<100 min),and the presence or absence of cells can be determined through color changes in the reaction system.The method has been verified by a large number of field samples and holds great feasibility,which is expected to be widely used in field monitoring of S.costatum.
作者
杨超
甄毓
秦铭俐
朱吉炜
YANG Chao;ZHEN Yu;QIN Mingli;ZHU Jiwei(Key Laboratory of Marine Environment and Ecology,Ministry of Education,College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Laboratory for Marine Ecology and Environmental Science,Pilot National Laboratory for Marine Science and Technology,Qingdao 266071,China;Ningbo Marine Center,Ministry of Natural Resources,Ningbo 315012,China)
出处
《海洋环境科学》
CAS
CSCD
北大核心
2024年第5期787-796,共10页
Marine Environmental Science
基金
国家重点研发计划课题(2022YFC3105202)
崂山实验室科技创新项目(LSKJ202203700)
宁波市自然资源和规划局项目(甬资规采合2023第56号)。
关键词
赤潮
中肋骨条藻
现场检测
环介导恒温扩增
red tide
Skeletonema costatum
in-situ detection
loop-mediated isothermal amplification
作者简介
杨超(1998-),男,天津人,硕士,研究方向为海洋环境科学,E-mail:az2282308571@163.com;通讯作者:甄毓(1978-),男,教授,硕士生导师,研究方向为海洋分子生态学,E-mail:zhenyu@ouc.edu.cn。
