摘要
微生物驱动的氨氧化过程是氧化亚氮(N2O)排放的重要来源.研究设施菜地长期种植对土壤氨氧化古菌(AOA)和氨氧化细菌(AOB)群落丰度和结构的变化,对评价土壤质量状况和温室气体转化方面具有重要意义.以河北省高邑县设施菜地为研究对象,采用分析化学方法和高通量测序技术,分别测定土壤理化性质、AOA和AOB群落组成及多样性,研究设施菜地不同种植年限(1、5、10和20 a)下土壤AOA和AOB群落变异及其养分驱动因子.结果表明,随设施菜地种植年限延长,土壤中全氮、有机质、碱解氮、速效磷和速效钾含量先增加后减少;硝态氮、铵态氮含量和电导率随着种植年限的延长先减少后增加;pH随着种植年限的延长不断降低.随着种植年限延长,土壤中AOA和AOB丰度和多样性都先减少后增加,所有年限AOA群落的优势纲主要是亚硝基菌纲(Nitrososphaeria)和未分类的奇古菌纲(unclassified Thaumarchaeota),优势属主要是亚硝酸念珠菌属(Candidatus Nitrosocaldus);AOB群落的优势纲主要是变形菌纲(Betaproteobacteria),优势属主要是亚硝化螺菌属(Nitrosospira).AOA的群落结构组成随种植年限的变化比AOB更明显.设施菜地种植年限延长所引起的土壤养分因子的变化与AOA和AOB的群落之间存在较为显著的相关关系,其中铵态氮、碱解氮和硝态氮是影响AOA群落的关键养分因子;电导率、速效钾和硝态氮是影响AOB群落的关键养分因子.设施菜地不同种植年限显著影响土壤中AOA和AOB丰度和群落结构.研究结果可为深入探索设施菜地土壤中温室气体转化及氮循环相关微生物机制提供理论依据.
The ammonia oxidation process driven by microorganisms is a dominant source for nitrous oxide(N2O)emissions.Here,we examined the influence of greenhouse vegetable planting ages on soil ammonia-oxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB),which is of great significance for assessing the soil quality status and greenhouse gas transformations.A field study was conducted at different times(1,5,10,and 20 a)in greenhouse vegetable soils of Gaoyi,Hebei Province.Chemical analysis and Illumina NovaSeq high-throughput sequencing were used to analyze the soil physicochemical properties and community structures and diversity of AOA and AOB.The variation in AOA and AOB communities and the driving factors in greenhouse soils at different ages were also investigated.The results showed that the contents of total nitrogen,organic matter,alkali-hydrolyzable nitrogen,available phosphorus,and available potassium first increased and then decreased with the prolongation of growth.The contents of nitrate nitrogen,ammonium nitrogen,and electrical conductivity first decreased and then increased with the prolongation of growth.The pH value of soils decreased with the prolongation of growth.The abundance and diversity index of AOA and AOB first decreased and then increased with the prolongation of growth.Nitrososphaeria,unclassified Thaumarchaeota,and Candidatus Nitrosocaldus were the dominant species of AOA,while Betaproteobacteria and Nitrosospira were the dominant species of AOB.The composition of the soil AOA community varied greatly compared to that of AOB with the prolongation of growth.Correlation analysis showed that the changes in soil nutrient factors had a significant correlation with AOA and AOB communities.Redundancy analysis indicated that ammonium nitrogen,alkali-hydrolyzable nitrogen,and nitrate nitrogen were key factors of AOA communities,while electrical conductivity,available potassium,and nitrate nitrogen were key factors for AOB.In summary,long-term planting of greenhouse vegetables significantly affected the abundance and composition of soil AOA and AOB communities.Our results provide a theoretical basis for further studies on the greenhouse gas transformation and microbial mechanisms of the nitrogen cycle in greenhouse soils.
作者
潘禹充
赵经纬
牛宏进
黄亚丽
王媛
张晓旭
PAN Yu-chong;ZHAO Jing-wei;NIU Hong-jin;HUANG Ya-li;WANG Yuan;ZHANG Xiao-xu(College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2024年第10期6077-6085,共9页
Environmental Science
基金
河北省自然科学基金项目(D2021208001)
河北省重点研发计划项目(22327503D)。
关键词
种植年限
设施菜地
氨氧化古菌
氨氧化细菌
群落结构
planting ages
greenhouse vegetable field
ammonia-oxidizing archaea
ammonia-oxidizing bacteria
community structure
作者简介
潘禹充(2000-),男,硕士研究生,主要研究方向为土壤氮素循环与温室气体减排,E-mail:18903371159@163.com;通信作者:张晓旭,E-mail:zhangxiaoxu@hebust.edu.cn。
