摘要
BACKGROUND Complement activation is recognized as an important factor in the progression of liver damage caused by acetaminophen(APAP).However,the role of the complement inhibitor C2-FH in APAP-induced liver injury remains unclear.AIM To explore C2-FH in protecting against APAP-induced liver injury by inhibiting complement activation.METHODS A model of APAP-induced liver injury was used to study the protective effect of C2-FH on liver injury.C2-FH was administered through intraperitoneal injection 30 minutes after APAP treatment.We detected the effects of C2-FH on liver function,inflammatory response and complement activation.Additionally,RNA-sequencing(RNA-Seq)analysis was conducted to understand the mechanism through which C2-FH provides protection against APAP-induced liver injury.RESULTS C2-FH inhibited the increase in serum alanine aminotransferase activity,aspartate aminotransferase activity and lactate dehydrogenase,and reduced liver tissue necrosis caused by APAP.Moreover,it attenuated the inflammatory response and inhibited complement activation in APAP-induced liver injury.RNA-Seq analysis provided additional explanations for the protective role of C2-FH against APAP-induced liver injury.CONCLUSION C2-FH attenuates APAP-induced liver injury by inhibiting complement activation.
基金
Supported by Natural Science Foundation of Guangxi,No.2020GXNSFDA238006
Special Fund of the Central Government Guiding Local Scientific and Technological Development by Guangxi Science and Technology Department,No.GuikeZY21195024
Research Enhancement Project for Junior Faculty in Higher Education Institutes of Guangxi,No.2018KY0419.
作者简介
Co-first author:Chun-Mei Li,ORCID number:0000-0002-4962-7133;Co-first author:Tian Sun;Co-corresponding authors:Chong Zhang;Corresponding author:Jun-Fei Jin,PhD,Professor,Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair,the Affiliated Hospital of Guilin Medical University,No.15 Lequn Road,Guilin 541001,Guangxi Zhuang Autonomous Region,China.changliangzijin@163.com,ORCID number:0000-0002-9918-0966.
