摘要
【目的】研究鞣花酸(ellagic acid,EA)对公猪精液常温保存过程中精子质量及抗氧化性能的影响,以期为鞣花酸在精液保存中的推广应用提供理论依据。【方法】试验采集4头2~3岁健康的成年杜洛克公猪精液,将合格精液分为5份,用添加不同浓度EA(0(对照)、5、10、15、20μg/mL)的BTS(Beltsville thawing solution)常温保存稀释液分别稀释,在保存的第1、3、5、7天检测精子活率、平均路径速度、侧摆幅度、鞭打频率、质膜完整率、顶体完整率及抗氧化能力等指标。【结果】精液保存第1天,15μg/mL EA组精子质膜完整率显著高于对照组(P<0.05),其他指标各试验组与对照组均无显著性差异(P>0.05)。精液保存第3天,10、15μg/mL EA组精子平均路径速度、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)显著高于对照组(P<0.05)。精液保存第5天,15、20μg/mL EA组精子侧摆幅度显著低于对照组及5、10μg/mL EA组(P<0.05),5、10、15μg/mL EA组精子活率显著高于对照组(P<0.05),10、15、20μg/mL EA组精液中CAT、谷胱甘肽过氧化物酶(GSH-Px)活性及T-AOC显著高于对照组(P<0.05)。精液保存第7天,各试验组精子活率、平均路径速度、质膜完整率、顶体完整率、CAT活性、T-AOC均显著高于对照组(P<0.05)。【结论】添加EA于BTS稀释液中,对常温保存的精液具有良好的保护作用,其中10μg/mL EA效果最好。
【Objective】The aim of this study was to investigate the effects of ellagic acid(EA)on the quality and antioxidant properties of boar semen during room temperature preservation,in order to provide a theoretical basis for the promotion and application of EA in semen preservation.【Method】Semen was collected from four healthy adult Duroc boars aged 2 to 3 years.The qualified semen was divided into five portions and diluted with BTS(Beltsville thawing solution)diluted at room temperature,which contained different concentrations of EA(0(control),5,10,15 and 20μg/mL).Sperm motility,average path velocity,swing amplitude,whipping frequency,plasma membrane integrity rate,acrosome integrity rate,and antioxidant capacity were assessed on the 1st,3rd,5th,and 7th days of preservation.【Result】On the 1st day of preservation,plasma membrane integrity was significantly higher in 15μg/mL EA group compared to control group(P<0.05),and there was no significant difference in other indicators among experimental groups and control group(P>0.05).On the 3rd day of semen preservation,the average path velocity,catalase(CAT)activity,and total antioxidant capacity(T-AOC)of sperm in 10 and 15μg/mL EA groups were significantly higher than those in control group(P<0.05).On the 5th day of semen preservation,the swing amplitude of sperm in 15 and 20μg/mL EA groups was significantly lower than that in control group and the 5 and 10μg/mL EA groups(P<0.05),and the sperm motility in 5,10 and 15μg/mL EA groups was significantly higher than that in control group(P<0.05).The CAT,glutathione peroxidase(GSH-Px)activitives,and T-AOC in the semen of 10,15 and 20μg/mL EA groups were significantly higher than those in control group(P<0.05).On the 7th day of semen preservation,the sperm motility,average path velocity,plasma membrane integrity,acrosome integrity,CAT activity,and T-AOC in each experimental group were significantly higher than those in control group(P<0.05).【Conclusion】The incorporation of EA into BTS dilution solution was found to offer a protective effect on semen during room-temperature storage,with the optimal outcome observed at an EA concentration of 10μg/mL.
作者
屈先先
舒亮
孙小晶
刘梅
高凤仙
杨泰
QU Xianxian;SHU Liang;SUN Xiaojing;LIU Mei;GAO Fengxian;YANG Tai(College of Animal Science and Technology,Hunan Agricultural University,Changsha 410000,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2024年第10期4383-4390,共8页
China Animal Husbandry & Veterinary Medicine
基金
湖南省重点研发计划项目(2023DK2004)。
关键词
鞣花酸
常温保存
精液品质
抗氧化能力
猪
ellagic acid
room temperature preservation
semen quality
antioxidant ability
pig
作者简介
屈先先,联系方式:E-mail:1551500616@qq.com;通信作者:高凤仙,E-mail:gaofx1964@163.com;通信作者:杨泰,E-mail:490669958@qq.com。
