摘要
建立了一种经QuEChERS EMR(Enhanced Matrix Removal)-Lipid净化结合超高效液相色谱-串联质谱(UHPLC-MS/MS)同时测定调味品中苏丹红Ⅰ~Ⅳ、对位红5种脂溶性色素和罂粟碱、吗啡、可待因、蒂巴因、那可丁5种罂粟壳生物碱残留量的高通量分析方法。罂粟碱、蒂巴因、那可丁和5种脂溶性色素在1~100 ng/mL范围内线性关系良好,相关系数均大于0.99,定量限(LOQ)为1.0μg/kg;吗啡、可待因在8~100 ng/mL范围内线性关系良好,相关系数均大于0.99,定量限(LOQ)为5.0μg/kg;各化合物平均回收率为81.2%~104.5%,相对标准偏差为2.9%~11.4%(n=6)。该方法简便、灵敏、稳定、通量高、成本低,适用于香辛料调味油、火锅底料及蘸料、麻辣烫底料等多种调味品的检测质控需求。
A high-throughput analysis method is established for the simultaneous determination of residual amount of five lipid soluble pigments such as Sudan RedⅠ~Ⅳand Para Red,as well as five poppy husk alkaloids such as papaverine,morphine,codeine,thebaine and narcotine in seasonings using QuEChERS EMR(Enhanced Matrix Removal)-Lipid purification combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Papaverine,thebaine,narcotine and five lipid soluble pigments show a good linear relationship within the range of 1~100 ng/mL,the correlation coefficients are all greater than 0.99,and the limit of quantification(LOQ)is 1.0μg/kg.Morphine and codeine show a good linear relationship within the range of 8~100 ng/mL,the correlation coefficients are both greater than 0.99,and the limit of quantification(LOQ)is 5.0μg/kg.The average recovery rates of each compound range from 81.2%to 104.5%,with the relative standard deviations between 2.9%and 11.4%(n=6).The method is simple,sensitive,stable,high-throughput and low-cost,and it is suitable for the detection and quality control requirements of various seasonings such as spice flavored oil,hotpot seasoning and dipping sauce,and Malatang seasoning.
作者
丁文慧
田洪霞
朱伟伟
张帅
DING Wen-hui;TIAN Hong-xia;ZHU Wei-wei;ZHANG Shuai(School of Food and Drug,Weifang Vocational College,Weifang 261000,China;Anqiu Inspection and Testing Center Co.,Ltd.,Weifang 262100,China)
出处
《中国调味品》
CAS
北大核心
2024年第10期179-185,共7页
China Condiment
基金
山东省重大科技创新工程项目(2019JZZY011014)
潍坊市科技发展计划项目(2021GX020)。
作者简介
丁文慧(1988-),女,讲师,博士,研究方向:食品加工与安全、教育管理学。
