摘要
该研究从动物的粪便中分离到一株产纳米硒贝莱斯芽孢杆菌(Bacillus velezensis),命名为N-8。该菌株在液体培养条件下其耐硒能力可达32 mmol/L。2 mmol/L硒处理条件下,N-8菌株可以在12 h之内将培养基中73.63%的亚硒酸钠转化为红色的纳米硒,72 h的转化效率达到90.54%,产生纳米硒的粒径为238.74 nm,在芽孢杆菌中属于高还原率菌株。同时还发现N-8对沙门氏菌、金黄色葡萄球菌、大肠杆菌3种典型的食源性病原菌表现出明显的抑制效果。全基因组测序和分析表明,Bacillus velezensis N-8基因组大小为3844241 bp,GC含量为45.61%,共编码3686个基因,基因组测序数据提交至NCBI获得GenBank登录为JAZIAW000000000。通过antiSMASH预测到N-8基因组中存在14种次级代谢产物生物合成相关的基因簇,其中7个与macrolactin H、bacillaene、bacillibactin、bacilysin、difficidin、fengycin、surfactin化合物合成相关基因簇完全一致或高度相似,推测以上次级代谢产物是N-8产生抑菌效果的主要原因。以上研究结果为富硒益生菌资源的开发和利用提供了参考和借鉴。
In this study,a nano-selenium-producing Bacillus velezensis strain was isolated from animal feces,designated as N-8.This strain demonstrated remarkable selenium tolerance,reaching up to 32 mmol/L under liquid culture conditions.When exposed to 2 mmol/L selenium treatment,strain N-8 converted 73.63%of the sodium selenite in the medium into red nano-selenium within 12 hours,with a conversion efficiency reaching 90.54%after 72 hours.The produced nano-selenium had a particle size of 238.74 nm,rendering it a high-reduction-rate strain among Bacillus species.It was also found that N-8 showed obvious inhibitory effect on Salmonella,Staphylococcus aureus and Escherichia coli,three typical food-borne pathogens.Whole-genome sequencing and analysis revealed that the Bacillus velezensis N-8 genome is 3844241 bp in size,with a GC content of 45.61%,encoding a total of 3686 genes.The genome sequencing data have been submitted to NCBI and assigned the GenBank accession number JAZIAW000000000.Through antiSMASH prediction,14 gene clusters related to the biosynthesis of secondary metabolites were identified in the N-8 genome,of which 7 were identical or highly similar to those involved in the synthesis of macrolactin H,bacillaene,bacillibactin,bacilysin,difficidin,fengycin,surfactin.It indicating that the above secondary metabolites are the main reason for the antimicrobial effect of N-8.These findings provide a reference for the development and utilization of selenium-rich probiotic resources.
作者
李佳栩
范诗悦
庞振斌
熊定红
罗以杰
马莹
程水源
董星星
LI Jiaxu;FAN Shiyue;PANG Zhenbin;XIONG Dinghong;LUO Yijie;MA Ying;CHENG Shuiyuan;DONG Xingxing(National R&D Center for Se-Rich Agricultural Products Processing,Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products,School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Zhongke Biotechnology Transfer(Shenzhen)Co.,Ltd.,Shenzhen 518000,China)
出处
《食品科技》
CAS
北大核心
2024年第8期1-10,共10页
Food Science and Technology
基金
湖北省自然科学基金项目(2022CFB945)
湖北省教育厅科学技术研究项目(Q20211612)
武汉轻工大学校企合作横向课题(whpu-2023-kj-2339)。
关键词
耐硒
贝莱斯芽孢杆菌
硒转化
基因组
selenium tolerance
Bacillus velezensis
selenium transformation
genome
作者简介
李佳栩(1998-),女,广西南宁人,硕士研究生,研究方向为富硒微生物资源开发与利用;通信作者:程水源;通信作者:董星星。
