摘要
【目的】丝氨酸乙酰转移酶(SAT)是硫同化为半胱氨酸(cysteine,Cys)的关键酶,参与植物多种生物过程,特别是在植物响应非生物胁迫中发挥着重要作用。但关于马铃薯SAT基因家族(StSAT)的分析尚未见报道。系统鉴定了马铃薯SAT基因家族,为深入了解StSAT基因家族的特征,进一步分析它们在马铃薯抵御非生物胁迫中的功能提供了理论依据。【方法】利用HMM对马铃薯SAT基因家族进行鉴定,并对其染色体分布、基因结构、蛋白保守基序及物种间的共线性进行分析。利用PGSC下载的RNA-seq数据分析双单倍体(doubled-monoploid,DM)马铃薯中StSATs在不同组织部位、非生物胁迫和外源激素处理下的表达模式。通过qPCR(quantitative real-time PCR)分析四倍体马铃薯中StSATs在NaCl和PEG处理(0、1、3和24 h)下的相对表达水平。【结果】在马铃薯中鉴定出4个StSATs,它们分布在4条染色体上。根据系统发育特征,将4个StSATs分在3个亚族中。共线性分析发现,StSATs与拟南芥(Arabidopsis thaliana)、番茄(Solanum lycopersicum)、甘蓝(Brassica oleracea)、水稻(Oryza sativa)和玉米(Zea mays)中分别有4对、4对、2对、1对和1对直系同源基因。通过表达分析发现,四倍体马铃薯中4个StSATs随着NaCl和PEG处理时间的延长,其表达量显著升高(与0 h相比),很可能参与马铃薯对盐和渗透胁迫的响应。【结论】StSAT基因家族成员在马铃薯响应盐和渗透胁迫中发挥着重要作用。
【Objective】Serine acetyltransferase(SAT)is a critical enzyme in assimilating sulfur into cysteine(Cys),participating in various biological processes in plants,especially in plant response to abiotic stress.However,analyses on the potato SAT gene family(StSAT)has not yet be reported.This study systematically and comprehensively identified the potato SAT gene family,providing a theoretical basis for further understanding the characteristics of the StSAT gene family and further analysis of their functions in potato resistance to abiotic stress.【Method】HMM was used to identify the StSAT gene family in potatoes and analyze their chromosomal distribution,gene structure,proteinconserved motifs,and collinearity across species.Additionally,RNA-seq data downloaded from PGSC were used to analyze the expression patterns of StSATs in different tissue parts of doubled-monoploid(DM)potatoes under abiotic stress and exogenous hormone treatments.qPCR(quantitative real-time PCR)was to analyze the relative expressions of StSATs in tetraploid potatoes under NaCl and PEG treatment(0,1,3,and 24 h)【Result】Four StSATs were identified in potatoes,and they were distributed across four chromosomes.Based on phylogenetic characteristics,these four StSATs were classified into three subfamilies.According to synteny analysis,there were 4 orthologous SAT genes to Arabidopsis,4 to tomato(Solanum lycopersicum),2 to cabbage(Brassica oleracea),1 to rice(Oryza sativa),and 1 to corn(Zea mays).Expression analysis showed that the expressions of all four StSATs significantly increased over time under NaCl and PEG treatments compared to 0 h,indicating their likely involvement in the potato's response to salt and osmotic stress.【Conclusion】StSAT gene family members play an essential role in potato responses to salt and osmotic stress.
作者
申鹏
高雅彬
丁红
SHEN Peng;GAO Ya-Bin;DING Hong(Lanzhou Bowen College of Science and Technology,Lanzhou 730101)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第9期64-73,共10页
Biotechnology Bulletin
基金
甘肃省教育厅高校教师创新基金项目(2023B-379)。
作者简介
申鹏,男,硕士,讲师,研究方向:园艺植物生物技术、植物修复和栽培生理,E-mail:lzbwkjxy_sp@126.com;通信作者:高雅彬,女,硕士,副教授,研究方向:园艺植物生物技术、环境保护及资源再生利用,E-mail:lzbwkjxy_gyb@126.com。
